Non-continuous Translation of Coat Protein and Ribonucleic Acid Polymerase Cistrons in MS2 Bacteriophage Ribonucleic Acid

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1974 Jan 1

PMID 4587604

Citations 1

Authors

M J Grubman

D Nakada

Affiliations

Soon will be listed here.

Abstract

In an MS2 phage ribonucleic acid (RNA)-directed in vitro protein-synthesizing system, the coat protein cistron and the adjacent RNA polymerase cistron are translated non-continuously. The ribosomes which have completed the synthesis of coat protein dissociate from the MS2 RNA and do not read through the intercistronic gap. Translation of the adjacent RNA polymerase cistron requires ribosomes other than those translating the coat protein cistron.

Citing Articles

Reinitiation of translation from the triplet next to the amber termination codon in the absence of ribosome-releasing factor.

Ryoji M, Berland R, Kaji A Proc Natl Acad Sci U S A. 1981; 78(10):5973-7.

PMID: 7031641 PMC: 348959. DOI: 10.1073/pnas.78.10.5973.

References

Adams J, Capecchi M . N-formylmethionyl-sRNA as the initiator of protein synthesis. Proc Natl Acad Sci U S A. 1966; 55(1):147-55. PMC: 285768. DOI: 10.1073/pnas.55.1.147. View

Weiner A, Weber K . Natural read-through at the UGA termination signal of Q-beta coat protein cistron. Nat New Biol. 1971; 234(50):206-9. DOI: 10.1038/newbio234206a0. View

Vinuela E, Algranati I, Feix G, Garwes D, Weissmann C, Ochoa S . Virus-specific proteins in Escherichia coli infected with some amber mutants of phage MS2. Biochim Biophys Acta. 1968; 155(2):558-65. DOI: 10.1016/0005-2787(68)90199-8. View

Sugiyama T, Nakada D . Translational control of bacteriophage MS2 RNA cistrons by MS2 coat protein: polyacrylamide gel electrophoretic analysis of proteins synthesized in vitro. J Mol Biol. 1968; 31(3):431-40. DOI: 10.1016/0022-2836(68)90419-1. View

Lodish H . Bacteriophage f2 RNA: control of translation and gene order. Nature. 1968; 220(5165):345-50. DOI: 10.1038/220345a0. View

SPAHR P, Farber M, GESTELAND R . Binding site on R17 RNA for coat protein. Nature. 1969; 222(5192):455-8. DOI: 10.1038/222455a0. View

Webster R, Zinder N . Fate of the message-ribosome complex upon translation of termination signals. J Mol Biol. 1969; 42(3):425-39. DOI: 10.1016/0022-2836(69)90234-4. View

Grubman M, Nakada D . Ribosomal subunits and MS2 phage RNA-directed protein synthesis. Nature. 1969; 223(5212):1242-7. DOI: 10.1038/2231242a0. View

Sugiyama T, Stone Jr H . Protein synthesis directed by an amber coat-protein mutant of the RNA phage MS2. J Mol Biol. 1969; 42(1):97-115. DOI: 10.1016/0022-2836(69)90489-6. View

10.

Lodish H, Robertson H . Cell-free synthesis of bacteriophage f2 maturation protein. J Mol Biol. 1969; 45(1):9-22. DOI: 10.1016/0022-2836(69)90206-x. View

11.

Steitz J . Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA. Nature. 1969; 224(5223):957-64. DOI: 10.1038/224957a0. View

12.

Nichols J . Nucleotide sequence from the polypeptide chain termination region of the coat protein cistron in bacteriophage R17 RNA. Nature. 1970; 225(5228):147-51. DOI: 10.1038/225147a0. View

13.

Jeppesen P, Steitz J, GESTELAND R, SPAHR P . Gene order in the bacteriophage R17 RNA: 5'-a protein-coat protein-synthetase-3'. Nature. 1970; 226(5242):230-7. DOI: 10.1038/226230a0. View

14.

Lodish H, Robertson H . Regulation of in vitro translation of bacteriophage f2 RNA. Cold Spring Harb Symp Quant Biol. 1969; 34:655-73. DOI: 10.1101/sqb.1969.034.01.076. View

15.

Capecchi M, Klein H . Release factors mediating termination of complete proteins. Nature. 1970; 226(5250):1029-33. DOI: 10.1038/2261029a0. View

16.

Sugiyama T, Nakada D . Translational control of bacteriophage MS2 RNA cistrons by MS2 coat protein: affinity and specificity of the interaction of MS2 coat protein with MS2 RNA. J Mol Biol. 1970; 48(2):349-55. DOI: 10.1016/0022-2836(70)90166-x. View

17.

Lodish H . Secondary structure of bacteriophage f2 ribonucleic acid and the initiation of in vitro protein biosynthesis. J Mol Biol. 1970; 50(3):689-702. DOI: 10.1016/0022-2836(70)90093-8. View

18.

Hsu W . Translation of an RNA viral message in vitro: one-step polypeptide chain elongation. Biochem Biophys Res Commun. 1971; 42(3):405-12. DOI: 10.1016/0006-291x(71)90385-8. View

19.

Lodish H . Thermal melting of bacteriophage f2 RNA and initiation of synthesis of the maturation protein. J Mol Biol. 1971; 56(3):627-32. DOI: 10.1016/0022-2836(71)90406-2. View

20.

Young R, Nakada D . Defective ribosomes in chloramphenicol-treated Escherichia coli. J Mol Biol. 1971; 57(3):457-73. DOI: 10.1016/0022-2836(71)90103-3. View