Replication of T4 DNA in Escherichia Coli Treated with Toluene

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1973 Dec 1

PMID 4586770

Citations 7

Authors

R C Miller Jr

D M Taylor

K MacKay

H W Smith

Affiliations

Soon will be listed here.

Abstract

Deoxyribonucleoside triphosphates are incorporated into T4 DNA in infected cells treated with toluene. Under the proper conditions the incorporation is controlled by the known T4 DNA polymerase and proceeds by a semiconservative mechanism. Both strands of the phage DNA are replicated into a high molecular weight progeny molecule. The replication system is accessible to extracellular pancreatic DNase added to the reaction mixture. At early times after infection a second replication system, not under control of the gene 43 polymerase, has been detected which synthesizes T4 DNA in toluenized cells.

Citing Articles

Molecular insights into the prototypical single-stranded DNA-binding protein from .

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Biochemistry of DNA-defective amber mutants of bacteriophage T4. IV. DNA synthesis in plasmolyzed cells.

Collinsworth W, Mathews C J Virol. 1974; 13(4):908-15.

PMID: 4595302 PMC: 355389. DOI: 10.1128/JVI.13.4.908-915.1974.

T4 DNA polymerase (gene 43) is required in vivo for repair of gaps in recombinants.

Miller Jr R J Virol. 1975; 15(2):316-21.

PMID: 1113374 PMC: 354455. DOI: 10.1128/JVI.15.2.316-321.1975.

Biological activity of T4 DNA synthesized in toluene-treated Escherichia coli cells.

McNicol L, Miller Jr R J Virol. 1975; 15(3):479-83.

PMID: 1089805 PMC: 354483. DOI: 10.1128/JVI.15.3.479-483.1975.

Properties of the nonlethal recombinational repair x and y mutants of bacteriophage T4. II. DNA synthesis.

Melamede R, Wallace S J Virol. 1977; 24(1):28-40.

PMID: 904025 PMC: 515907. DOI: 10.1128/JVI.24.1.28-40.1977.

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