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A Cis-trans Test of the Effect of the First Enzyme for Histidine Biosynthesis on Regulation of the Histidine Operon

Overview
Journal J Bacteriol
Publisher American Society for Microbiology
Specialty Microbiology
Date 1973 Apr 1
PMID 4572718
Citations 11
Authors
J S Kovach
A O Ballesteros
M Meyers
M Soria
R F GOLDBERGER
Affiliations
Soon will be listed here.
Abstract

Previous studies showed that when triazolalanine was added to a derepressed culture of a histidine auxotroph, repression of the histidine operon occurred as though histidine had been added (6). However, when triazolalanine was added to a derepressed culture of a strain with a mutation in the first gene of the histidine operon which rendered the first enzyme for histidine biosynthesis resistant to inhibition by histidine, repression did not occur. The studies reported here represent a cis/trans test of this effect of mutations to feedback resistance. Using specially constructed merodiploid strains, we were able to show that the wild-type allele is dominant to the mutant (feedback resistant) allele and that the effect operates in trans. We conclude that the enzyme encoded by the first gene of the histidine operon exerts its regulatory effect on the operon not by acting locally at its site of synthesis, but by acting as a freely diffusible protein.

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References
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