Physical Assay of Competence for Specific Mating-pair Formation in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Apr 1

PMID 4572707

Citations 7

Authors

R H Walmsley

Affiliations

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Abstract

The fractions of Escherichia coli female and male bacteria that are competent to form specific mating pairs at any time during normal exponential growth have been measured by a physical assay. The competencies of a Hayes- and a Cavali-type donor were 35 and 50%, respectively, in agreement with piliation assays reported by others. The competencies of two recipient strains, PA309 and P678, were found to be 33 and 29%, respectively.

Citing Articles

Con--mutants: class of mutants in Escherichia coli K-12 lacking a major cell wall protein and defective in conjugation and adsorption of a bacteriophage.

Skurray R, Hancock R, Reeves P J Bacteriol. 1974; 119(3):726-35.

PMID: 4604263 PMC: 245674. DOI: 10.1128/jb.119.3.726-735.1974.

Conjugal deoxyribonucleic acid replication by Excherichia coli K-12: effect of chloramphenicol and rifampin.

Fenwick Jr R, Curtiss 3rd R J Bacteriol. 1973; 116(3):1224-35.

PMID: 4584805 PMC: 246478. DOI: 10.1128/jb.116.3.1224-1235.1973.

Mating aggregates in Escherichia coli conjugation.

Achtman M J Bacteriol. 1975; 123(2):505-15.

PMID: 1097414 PMC: 235755. DOI: 10.1128/jb.123.2.505-515.1975.

Characterization of an Escherichia coli K-12 F-Con-mutant.

Havekes L, HOEKSTRA W J Bacteriol. 1976; 126(2):593-600.

PMID: 770448 PMC: 233191. DOI: 10.1128/jb.126.2.593-600.1976.

Temperature dependence of mating-pair formation in Escherichia coli.

Walmsley R J Bacteriol. 1976; 126(1):222-4.

PMID: 770422 PMC: 233278. DOI: 10.1128/jb.126.1.222-224.1976.

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