Plasmid-associated Functions of a Stable Flac

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1973 Jan 1

PMID 4569402

Citations 5

Authors

F L Macrina

E Balbinder

Affiliations

Soon will be listed here.

Abstract

Several functions associated with the stable plasmid, FlacS, have been examined. Our results indicate that the sex pili synthesized by Escherichia coli strains carrying FlacS are altered in some manner as evidenced by a very inefficient adsorption of male-specific phages. On the other hand, FlacS-mediated entry exclusion of related plasmids and plasmid incompatibility function(s) appear normal. The presence of covalently closed circular deoxyribonucleic acid in E. coli strains harboring FlacS indicates that it is an autonomously replicating plasmid. Based on beta-galactosidase levels and the percentage of covalently closed circular deoxyribonucleic acid, it appears that the stability of the FlacS is not the result of multiple copies of this plasmid. FlacS appears larger than its precursor, F(ts114)lac, in sedimentation through alkaline sucrose gradients.

Citing Articles

Molecular studies of an fi+ plasmid from strains of Salmonella typhimurium.

Smith H, Humphreys G, Grindley N, Grindley J, Anderson E Mol Gen Genet. 1973; 126(2):143-51.

PMID: 4591377 DOI: 10.1007/BF00330989.

Evidence for a composite state of an F'his, gnd element and a cryptic plasmid in a derivative of Salmonella typhimurium LT2.

Oliver D, Manis J, Whitfield H J Bacteriol. 1974; 119(1):192-201.

PMID: 4366251 PMC: 245590. DOI: 10.1128/jb.119.1.192-201.1974.

Isolation of a hybrid F' factor-carrying Escherichia coli lactose region and Salmonella typhimurium histidine region, F42-400 (F' ts114 lac+, his+): its partial characterization and behavior in Salmonella typhimurium.

Rao R, Pereira M J Bacteriol. 1975; 123(3):779-91.

PMID: 1099076 PMC: 235798. DOI: 10.1128/jb.123.3.779-791.1975.

Linkage map of Salmonella typhimurium, edition V.

Sanderson K, Hartman P Microbiol Rev. 1978; 42(2):471-519.

PMID: 353483 PMC: 281437. DOI: 10.1128/mr.42.2.471-519.1978.

An effect of F-like plasmids on the maintenance of Flac in a dnaC mutant of Salmonella typhimurium.

Lemoine V, Rowbury R Mol Gen Genet. 1977; 156(3):313-8.

PMID: 340909 DOI: 10.1007/BF00267187.

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