Self-transfer and Genetic Recombination Mediated by P, the Sex Factor of Vibrio Cholerae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 Nov 1

PMID 4563971

Citations 19

Authors

C Parker

W R Romig

Affiliations

Soon will be listed here.

Abstract

Vibrio cholerae cells, infected with the sex factor P, produce discrete, plaque-like clearings when plated on lawns of P(-) cells. We investigated the nature of these clearings and conclude that they are probably sites of active mating. We developed a quantitative assay for P(+) cells and used it to study the kinetics of sex factor spread in broth cultures. Both established and newly infected donor populations were efficient sex factor donors, indicating that P is not self-repressed. We also investigated the kinetics of recombinant formation in broth matings. In 1-hr matings, we routinely found recombination frequencies of 10(-6) per donor cell. Kinetic studies of recombinant formation showed that the markers tested all appeared at early times. Thus P, the V. cholerae sex factor, seems to resemble F in its transfer properties.

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References

BRINTON Jr C . The properties of sex pili, the viral nature of "conjugal" genetic transfer systems, and some possible approaches to the control of bacterial drug resistance. CRC Crit Rev Microbiol. 1971; 1(1):105-60. DOI: 10.3109/10408417109104479. View

Newman F, EISENSTARK A . PHAGE-HOST RELATIONSHIPS IN VIBRIO CHOLERAE. J Infect Dis. 1964; 114:217-25. DOI: 10.1093/infdis/114.3.217. View

Watanabe T . EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE IN ENTEROBACTERIACEAE. VI. HIGH-FREQUENCY RESISTANCE TRANSFER SYSTEM IN ESCHERICHIA COLI. J Bacteriol. 1963; 85:788-94. PMC: 278226. DOI: 10.1128/jb.85.4.788-794.1963. View

Pierce N, Greenough 3rd W, Carpenter Jr C . Vibrio cholerae enterotoxin and its mode of action. Bacteriol Rev. 1971; 35(1):1-13. PMC: 378369. DOI: 10.1128/br.35.1.1-13.1971. View

Takeya K, Shimodori S . New method for the detection of a lethal factor in vibrios. J Bacteriol. 1969; 99(1):339-40. PMC: 250008. DOI: 10.1128/jb.99.1.339-340.1969. View

Bhaskaran K, Dyer P, ROGERS G . Sex pili in Vibrio cholerae. Aust J Exp Biol Med Sci. 1969; 47(5):647-50. DOI: 10.1038/icb.1969.160. View

Bhaskaran K, Sinha V . Transmissible plasmid factors and fertility inhibition in Vibrio cholerae. J Gen Microbiol. 1971; 69(1):89-97. DOI: 10.1099/00221287-69-1-89. View

Bhaskaran K . Genetic recombination in Vibrio cholerae. J Gen Microbiol. 1958; 19(1):71-5. DOI: 10.1099/00221287-19-1-71. View

Bhaskaran K . SEGREGATION OF GENETIC FACTORS DURING RECOMBINATION IN VIBRIO CHOLERAE, STRAIN 162. Bull World Health Organ. 1964; 30:845-53. PMC: 2555083. View

10.

DEMEREC M, Adelberg E, Clark A, Hartman P . A proposal for a uniform nomenclature in bacterial genetics. Genetics. 1966; 54(1):61-76. PMC: 1211113. DOI: 10.1093/genetics/54.1.61. View

11.

Curtiss 3rd R, Renshaw J . F+ strains of Escherichia coli K-12 defective in Hfr formation. Genetics. 1969; 63(1):7-26. PMC: 1212339. DOI: 10.1093/genetics/63.1.7. View

12.

Enomoto M, Iino T . COLONIAL DIMORPHISM IN NONMOTILE SALMONELLA. J Bacteriol. 1963; 86:473-7. PMC: 278458. DOI: 10.1128/jb.86.3.473-477.1963. View

13.

Bhaskaran K . Observations of the nature of genetic recombination in Vibrio cholerae. Indian J Med Res. 1959; 47(3):253-60. View

14.

Lacey R . Transfer of tetracycline-resistance between strains of Staphylococcus aureus in mixed cultures. J Gen Microbiol. 1971; 69(2):229-37. DOI: 10.1099/00221287-69-2-229. View

15.

Bhaskaran K . Recombination of characters between mutant stocks of Vibrio cholerae, strain 162. J Gen Microbiol. 1960; 23:47-54. DOI: 10.1099/00221287-23-1-47. View

16.

Parker C, Richardson S, Romig W . Production of Bacteriophage-Associated Materials by Vibrio cholerae: Possible Correlation with Pathogenicity. Infect Immun. 1970; 1(4):417-20. PMC: 415914. DOI: 10.1128/iai.1.4.417-420.1970. View

17.

Watanabe T, Fukasawa T . Episome-mediated transfer of drug resistance in Enterobacteriaceae IV. Interactions between resistance transfer factor and F-factor in Escherichia coli K-12. J Bacteriol. 1962; 83:727-35. PMC: 279346. DOI: 10.1128/jb.83.4.727-735.1962. View