Primary Structure of Neocarzinostatin, an Antitumor Protein

Overview

Journal Science

Specialty Science

Date 1972 Nov 24

PMID 4563817

Citations 13

Authors

J Meienhofer

H Maeda

C B Glaser

J CZOMBOS

K Kuromizu

Affiliations

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Abstract

The antitumor protein neocarzinostatin is an acidic single-chain molecule, cross-linked by two disulfide bridges, and consists of 109 amino acid residues. Complete disulfide bond reduction and S-carboxymethylation was achieved in liquid ammonia. Sequence determination of five tryptic fragments led to the proposed primary structure.

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Identification of thymidine-5'-aldehyde at DNA strand breaks induced by neocarzinostatin chromophore.

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Roles of chromophore and apo-protein in neocarzinostatin action.

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