Methionine Adenosyltransferase and Ethionine Resistance in Saccharomyces Cerevisiae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 Sep 1

PMID 4559828

Citations 13

Authors

J E Mertz

K D Spence

Affiliations

Soon will be listed here.

Abstract

The methionine adenosyltransferase is repressed in Saccharomyces cerevisiae during growth in the presence of excess methionine. The relationship of this repression to the level of intracellular S-adenosylmethionine is discussed. In conjunction with these studies, an ethionine-resistant mutant has been investigated which has a low level of methionine adenosyltransferase under all conditions tested. The mechanism of ethionine resistance in the latter strain apparently depends on its inability to form large quantities of intracellular S-adenosylethionine. With respect to the methionine adenosyltransferase, there is no apparent interaction between ethionine-resistant and ethionine-sensitive alleles when both are present in the heterozygous diploid.

Citing Articles

Effect of Methionine on Gene Expression in Cells.

Ianshina T, Sidorin A, Petrova K, Shubert M, Makeeva A, Sambuk E Microorganisms. 2023; 11(4).

PMID: 37110303 PMC: 10143545. DOI: 10.3390/microorganisms11040877.

Characterization of soybean tissue culture cell lines resistant to methionine analogs.

Madison J, Thompson J Plant Cell Rep. 2013; 7(7):473-6.

PMID: 24240394 DOI: 10.1007/BF00272734.

A dominant negative effect of eth-1r, a mutant allele of the Neurospora crassa S-adenosylmethionine synthetase-encoding gene conferring resistance to the methionine toxic analogue ethionine.

Barra J, Mautino M, Rosa A Genetics. 1996; 144(4):1455-62.

PMID: 8978034 PMC: 1207698. DOI: 10.1093/genetics/144.4.1455.

Inhibition of leucine transport in Saccharomyces by S-adenosylmethionine.

Law R, Ferro A J Bacteriol. 1980; 143(1):427-31.

PMID: 6995442 PMC: 294262. DOI: 10.1128/jb.143.1.427-431.1980.

Stimulation of yeast ascospore germination and outgrowth by S-adenosylmethionine.

Brawley J, Ferro A J Bacteriol. 1980; 142(2):608-14.

PMID: 6991481 PMC: 294033. DOI: 10.1128/jb.142.2.608-614.1980.

References

KAPPY M, Metzenberg R . Studies on the basis of ethionine-resistance in Neurospora. Biochim Biophys Acta. 1965; 107(3):425-33. DOI: 10.1016/0304-4165(65)90186-8. View

SURDIN Y, Sly W, Sire J, Bordes A, ROBICHON-SZULMAJSTER H . [Properties and genetic control of the system for accumulation of amino acids in Saccharomyces cerevisiae]. Biochim Biophys Acta. 1965; 107(3):546-66. View

SCHLENK F, Zydek C, EHNINGER D, DAINKO J . The production of S-adenosyl-L-methionine and S-adenosyl-L-ethionine by yeast. Enzymologia. 1965; 29(3):283-98. View

SHAPIRO S, EHNINGER D . Methods for the analysis and preparation of adenosylmethionine and adenosylhomocysteine. Anal Biochem. 1966; 15(2):323-33. DOI: 10.1016/0003-2697(66)90038-8. View

MAW G . Incorporation and distribution of ethionine-sulfur in the protein of ethionine-sensitive and ethionine-resistant yeasts. Arch Biochem Biophys. 1966; 115(2):291-301. DOI: 10.1016/0003-9861(66)90277-3. View

Pan F, TARVER H . Comparative studies on methionine, selenomethionine, and their ethyl analogues as substrates for methionine adenosyltransferase from rat liver. Arch Biochem Biophys. 1967; 119(1):429-34. DOI: 10.1016/0003-9861(67)90474-2. View

Spence K, Parks L, SHAPIRO S . Dominant mutation for ethionine resistance in Saccharomyces cerevisae. J Bacteriol. 1967; 94(5):1531-7. PMC: 276860. DOI: 10.1128/jb.94.5.1531-1537.1967. View

Groves W, Davis Jr F, SELLS B . Spectrophotometric determination of microgram quantities of protein without nucleic acid interference. Anal Biochem. 1968; 22(2):195-210. DOI: 10.1016/0003-2697(68)90307-2. View

Cherest H, Talbot G, ROBICHON-SZULMAJSTER H . Methionine biosynthesis from the 4-carbon skeleton of ethionine in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1968; 32(4):723-30. DOI: 10.1016/0006-291x(68)90299-4. View

10.

Cherest H, Eichler F, ROBICHON-SZULMAJSTER H . Genetic and regulatory aspects of methionine biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1969; 97(1):328-36. PMC: 249604. DOI: 10.1128/jb.97.1.328-336.1969. View

11.

Cox R, Smith R . Inhibition of S-adenosylmethionine formation by analogues of methionine. Arch Biochem Biophys. 1969; 129(2):615-9. DOI: 10.1016/0003-9861(69)90222-7. View

12.

GREENE R . Kinetic studies of the mechanism of S-adenosylmethionine synthetase from yeast. Biochemistry. 1969; 8(6):2255-65. DOI: 10.1021/bi00834a004. View

13.

GREENE R, Su C, Holloway C . S-Adenosylmethionine synthetase deficient mutants of Escherichia coli K-12 with impaired control of methionine biosynthesis. Biochem Biophys Res Commun. 1970; 38(6):1120-6. DOI: 10.1016/0006-291x(70)90355-4. View

14.

Cherest H, Talbot G, ROBICHON-SZULMAJSTER H . Role of homocysteine synthetase in an alternate route for methionine biosynthesis in Saccharomyces cerevisiae. J Bacteriol. 1970; 102(2):448-61. PMC: 247570. DOI: 10.1128/jb.102.2.448-461.1970. View

15.

Holloway C, GREENE R, Su C . Regulation of S-adenosylmethionine synthetase in Escherichia coli. J Bacteriol. 1970; 104(2):734-47. PMC: 285052. DOI: 10.1128/jb.104.2.734-747.1970. View

16.

Spence K . Mutation of Saccharomyces cerevisiae preventing uptake of S-adenosylmethionine. J Bacteriol. 1971; 106(2):325-30. PMC: 285100. DOI: 10.1128/jb.106.2.325-330.1971. View

17.

CANTONI G . S-Adenosylmethionine; a new intermediate formed enzymatically from L-methionine and adenosinetriphosphate. J Biol Chem. 1953; 204(1):403-16. View

18.

CANTONI G, Durell J . Activation of methionine for transmethylation. II. The methionine-activating enzyme; studies on the mechanism of the reaction. J Biol Chem. 1957; 225(2):1033-48. View

19.

SCHLENK F, DEPALMA R . The formation of S-adenosylmethionine in yeast. J Biol Chem. 1957; 229(2):1037-50. View

20.

Mudd S, CANTONI G . Activation of methionine for transmethylation. III. The methionine-activating enzyme of Bakers' yeast. J Biol Chem. 1958; 231(1):481-92. View