In Vitro Synthesis of Enzymes of the Tryptophan Operon of Escherichia Coli

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Jul 1

PMID 4558657

Citations 14

Authors

P H Pouwels

J van Rotterdam

Affiliations

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Abstract

Two active enzymes of the tryptophan operon of E. coli, anthranilate synthetase (product of trp E and trp D) and tryptophan synthetase (EC 4.2.1.20) (product of trp B and trp A), have been synthesized in a DNA-dependent preparation for protein synthesis. Anthranilate synthetase and the two components of tryptophan synthetase (tryptophan synthetase A and B) are made in vitro in equimolar amounts, suggesting that regulation of the translation is similar in vivo and in vitro. Kinetics of the synthesis of the two enzymes suggest that transcription and translation proceed in vitro in a correct temporal sequence.

Citing Articles

In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli. Evidence for positive control of transcription.

Pouwels P, van Rotterdam J Mol Gen Genet. 1975; 136(3):215-26.

PMID: 16094972 DOI: 10.1007/BF00334016.

Punctuation of transcription in vitro of the tryptophan operon of Escherichia coli. A novel type of control of transcription.

Pannekoek H, Brammar W, Pouwels P Mol Gen Genet. 1975; 136(3):199-214.

PMID: 16094971 DOI: 10.1007/BF00334015.

In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli.

Pouwels P, van Rotterdam J Mol Gen Genet. 1975; 136(3):185-97.

PMID: 16094970 DOI: 10.1007/BF00334014.

Expression of the trp operon in phi80trp transducing phages. Orientation of transcription and an artificial high-efficiency promotor in phage lambda h +phi80 pt5-2AB.

Pouwels P, Stevens W Mol Gen Genet. 1973; 120(1):55-68.

PMID: 4686211 DOI: 10.1007/BF00332984.

Expression of the tryptophan operon in merodiploids of Escherichia coli. II. Effects of polar mutations in the trpE gene.

SOMERVILLE R, Stetson H Mol Gen Genet. 1974; 131(3):247-61.

PMID: 4612330 DOI: 10.1007/BF00267964.

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