Specific Endonuclease R Fragments of Bacteriophage PhiX174 Deoxyribonucleic Acid

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1972 Apr 1

PMID 4553678

Citations 39

Authors

M H Edgell

C A HUTCHISON 3rd

M Sclair

Affiliations

Soon will be listed here.

Abstract

The restriction enzyme from Hemophilus influenzae, endonuclease R, cleaves phiX174 replicative-form deoxyribonucleic acid (DNA) into at least 13 specific limit fragments. The molecular weights of 12 of the fragments have been estimated by gel electrophoresis and electron microscopy. Using the genetic assay for small fragments of phiX DNA, we have shown that we can salvage markers from the endonuclease R phiX-RF fragments.

Citing Articles

Inverse PCR for Site-Directed Mutagenesis.

Silva D, Santos G, Barroca M, Costa D, Collins T Methods Mol Biol. 2023; 2967:223-238.

PMID: 37608115 DOI: 10.1007/978-1-0716-3358-8_18.

Cleavage of bacteriophage M13 DNA by Haemophilus influenzae endonuclease-R.

Van den Hondel C, Schoenmakers J Mol Biol Rep. 2013; 1(1):41-5.

PMID: 24197429 DOI: 10.1007/BF00357404.

Sites of cleavage by restriction enzymes in viral DNAs: comparison of observed and expected frequencies.

Elton R Nucleic Acids Res. 1974; 1(10):1343-50.

PMID: 10793694 PMC: 344355. DOI: 10.1093/nar/1.10.1343.

Cloning single-stranded DNA.

Messing J Mol Biotechnol. 1996; 5(1):39-47.

PMID: 8853015 DOI: 10.1007/BF02762411.

Site-specific mutagenesis on cloned DNAs: generation of a mutant of Escherichia coli tyrosine suppressor tRNA in which the sequence G-T-T-C corresponding to the universal G-T-pseudouracil-C sequence of tRNAs is changed to G-A-T-C.

Kudo I, Leineweber M, RajBhandary U Proc Natl Acad Sci U S A. 1981; 78(8):4753-7.

PMID: 6170979 PMC: 320241. DOI: 10.1073/pnas.78.8.4753.

References

Arber W, Linn S . DNA modification and restriction. Annu Rev Biochem. 1969; 38:467-500. DOI: 10.1146/annurev.bi.38.070169.002343. View

Benbow R, Hutchison C, Fabricant J, SINSHEIMER R . Genetic Map of Bacteriophage phiX174. J Virol. 1971; 7(5):549-58. PMC: 356162. DOI: 10.1128/JVI.7.5.549-558.1971. View

GUTHRIE G, SINSHEIMER R . Observations on the infection of bacterial protoplasts with the deoxyribonucleic acid of bacteriophage phi X174. Biochim Biophys Acta. 1963; 72:290-7. View

Shapiro A, Vinuela E, Maizel Jr J . Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967; 28(5):815-20. DOI: 10.1016/0006-291x(67)90391-9. View

HUTCHISON 3rd C, Edgell M . Genetic assay for small fragments of bacteriophage phi X174 deoxyribonucleic acid. J Virol. 1971; 8(2):181-9. PMC: 356229. DOI: 10.1128/JVI.8.2.181-189.1971. View

Weisbeek P, VAN DE POL J . Bological activity of phi X174 replicative form DNA fragments. Biochim Biophys Acta. 1970; 224(2):328-38. View

CENTER M, Studier F, Richardson C . The structural gene for a T7 endonuclease essential for phage DNA synthesis. Proc Natl Acad Sci U S A. 1970; 65(1):242-8. PMC: 286216. DOI: 10.1073/pnas.65.1.242. View

Burton A, SINSHEIMER R . The process of infection with bacteriophage phi-X174 VII. Ultracentrifugal analysis of the replicative form. J Mol Biol. 1965; 14(2):327-47. DOI: 10.1016/s0022-2836(65)80185-1. View

Peacock A, DINGMAN C . Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968; 7(2):668-74. DOI: 10.1021/bi00842a023. View

10.

Bishop D, Claybrook J, Spiegelman S . Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967; 26(3):373-87. DOI: 10.1016/0022-2836(67)90310-5. View

11.

Smith H, WILCOX K . A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970; 51(2):379-91. DOI: 10.1016/0022-2836(70)90149-x. View

12.

Hudson B, Upholt W, Devinny J, Vinograd J . The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA. Proc Natl Acad Sci U S A. 1969; 62(3):813-20. PMC: 223671. DOI: 10.1073/pnas.62.3.813. View

13.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View

14.

Davis R, Davidson N . Electron-microscopic visualization of deletion mutations. Proc Natl Acad Sci U S A. 1968; 60(1):243-50. PMC: 539109. DOI: 10.1073/pnas.60.1.243. View

15.

Schnegg B, Hofschneider P . Mutant of phi X174 accessible to host-controlled modification. J Virol. 1969; 3(5):541-2. PMC: 375809. DOI: 10.1128/JVI.3.5.541-542.1969. View

16.

Lindqvist B, SINSHEIMER R . Process of infection with bacteriophage phi-X174. XIV. Studies on macromolecular synthesis during infection with a lysis-defective mutant. J Mol Biol. 1967; 28(1):87-94. DOI: 10.1016/s0022-2836(67)80079-2. View

17.

Kelly Jr T, Smith H . A restriction enzyme from Hemophilus influenzae. II. J Mol Biol. 1970; 51(2):393-409. DOI: 10.1016/0022-2836(70)90150-6. View

18.

Meselson M, Yuan R . DNA restriction enzyme from E. coli. Nature. 1968; 217(5134):1110-4. DOI: 10.1038/2171110a0. View

19.

Funk F, SINSHEIMER R . Process of infection with bacteriophage phiX174. XXXV. Cistron 8. J Virol. 1970; 6(1):12-9. PMC: 376084. DOI: 10.1128/JVI.6.1.12-19.1970. View

20.

Cohen S, Miller C . Multiple molecular species of circular R-factor DNA isolated from Escherichia coli. Nature. 1969; 224(5226):1273-7. DOI: 10.1038/2241273a0. View