Effect of Drugs on Deoxyribonucleic Acid Synthesis in Isolated Mammalian Cell Nuclei. Comparison with Partially Purified Deoxyribonucleic Acid Polymerases

Overview

Journal Biochem J

Specialty Biochemistry

Date 1979 Mar 15

PMID 454371

Citations 6

Authors

J F Burke

P M Duff

C K Pearson

Affiliations

Soon will be listed here.

Abstract

In order to ascertain the identity of the DNA-dependent DNA polymerase responsible for the observed DNA synthesis in nuclei isolated from baby-hamster kidney (BHK-21/C13) cells a comparative study was carried out on the effects of some drugs, reported to influence DNA synthesis, on DNA synthesis catalysed by these nuclei and by partially purified DNA polymerase-alpha and -beta. In all cases DNA synthesis by isolated nuclei and polymerase-alpha was inhibited to similar extents by N-ethylmaleimide, p-hydroxymercuribenzoate, novobiocin, heparin and phosphonoacetic acid; polymerase-beta was much less affected by these compounds. Ethidium bromide inhibited all DNA synthesis to similar extents, although at low concentrations (about 2 microgram/ml) synthesis in isolated nuclei was stimulated. The results are discussed in relation to the proposal that DNA polymerase-alpha catalyses the covalent extension of Okazaki fragments that these nuclei carry out in vitro.

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