Ribonucleic Acid from Aerobically and Anaerobically Grown Rhodopseudomonas Spheroides: Comparison by Hybridization to Chromosomal and Satellite Deoxyribonucleic Acid

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1972 May 1

PMID 4537109

Citations 2

Authors

S S Witkin

K D Gibson

Affiliations

Soon will be listed here.

Abstract

Ribonucleic acid (RNA) species from aerobically and anaerobically grown Rhodopseudomonas spheroides were compared via hybridization to deoxyribonucleic acid (DNA). Both long-labeled and stable RNA bound to chromosomal DNA to the same extent, regardless of derivation. About 4% of the chromosomal DNA hybridized with total cell RNA and about 0.08% with stable RNA. About 4% of the mixed satellite DNA could be hybridized to total cell RNA from aerobic or anaerobic cultures, whereas essentially no stable RNA formed a hybrid with this DNA. Hybridization competition experiments with aerobic and anaerobic pulse-labeled RNA and chromosomal or satellite DNA demonstrated that no qualitative differences existed between the RNA species. It is concluded that identical species of RNA in the same relative amounts are synthesized by R. spheroides during aerobic or anaerobic growth on the same medium.

Citing Articles

Variation of levels of mRNA coding for antenna and reaction center polypeptides in Rhodopseudomonas capsulata in response to changes in oxygen concentration.

Clark W, Davidson E, Marrs B J Bacteriol. 1984; 157(3):945-8.

PMID: 6199343 PMC: 215353. DOI: 10.1128/jb.157.3.945-948.1984.

Genetics of Rhodospirillaceae.

Saunders V Microbiol Rev. 1978; 42(2):357-84.

PMID: 353480 PMC: 281434. DOI: 10.1128/mr.42.2.357-384.1978.

References

Okano P, Bacon K, Rosenberg E . Ribonucleic acid synthesis during microcyst formation in Myxococcus xanthus: characterization by deoxyribonucleic acid-ribonucleic acid hybridization. J Bacteriol. 1970; 104(1):275-82. PMC: 248211. DOI: 10.1128/jb.104.1.275-282.1970. View

Studier F, Maizel Jr J . T7-directed protein synthesis. Virology. 1969; 39(3):575-86. DOI: 10.1016/0042-6822(69)90105-6. View

LAVALLE R . Regulation at the level of translation in the arginine pathway of Escherichia coli K12. J Mol Biol. 1970; 51(2):449-51. DOI: 10.1016/0022-2836(70)90154-3. View

Segen B, Gibson K . Deficiencies of chromatophore proteins in some mutants of Rhodopseudomonas spheroides with altered carotenoids. J Bacteriol. 1971; 105(3):701-9. PMC: 248489. DOI: 10.1128/jb.105.3.701-709.1971. View

Aloni Y, Attardi G . Expression of the mitochondrial genome in HeLa cells. II. Evidence for complete transcription of mitochondrial DNA. J Mol Biol. 1971; 55(2):251-67. DOI: 10.1016/0022-2836(71)90195-1. View

Sueoka N, KANO-SUEOKA T . Transfer RNA and cell differentiation. Prog Nucleic Acid Res Mol Biol. 1970; 10:23-55. DOI: 10.1016/s0079-6603(08)60560-7. View

Kennell D . Principles and practices of nucleic acid hybridization. Prog Nucleic Acid Res Mol Biol. 1971; 11:259-301. DOI: 10.1016/s0079-6603(08)60330-x. View

Wilcox G, Singer J, Heffernan L . Deoxyribonucleic acid-ribonucleic acid hybridization studies on the L-Arabinose operon of Escherichia coli B-r. J Bacteriol. 1971; 108(1):1-4. PMC: 247024. DOI: 10.1128/jb.108.1.1-4.1971. View

Witkin S, Gibson K . Changes in ribonucleic acid turnover during aerobic and anaerobic growth in Rhodopseudomonas spheroides. J Bacteriol. 1972; 110(2):677-83. PMC: 247464. DOI: 10.1128/jb.110.2.677-683.1972. View

10.

Summers W, Jakes K . Phage T7 lysozyme mRNA transcription and translation in vivo and in vitro. Biochem Biophys Res Commun. 1971; 45(2):315-20. DOI: 10.1016/0006-291x(71)90820-5. View

11.

Gibson K, Niederman R . Characterization of two circular satellite species of deoxyribonucleic acid in Rhodopseudomonas spheroides. Arch Biochem Biophys. 1970; 141(2):694-704. DOI: 10.1016/0003-9861(70)90190-6. View

12.

LAVALLE R, DE HAUWER G . Tryptophan messenger translation in Escherichia coli. J Mol Biol. 1970; 51(2):435-47. DOI: 10.1016/0022-2836(70)90153-1. View

13.

Brown R, Haselkorn R . Chloroplast RNA populations in dark-grown, light-grown, and greening Euglena gracilis. Proc Natl Acad Sci U S A. 1971; 68(10):2536-9. PMC: 389463. DOI: 10.1073/pnas.68.10.2536. View

14.

Yamashita J, Kamen M . Observations on the nature of pulse-labeled RNA's from photosynthetically or heterotrophically grown Rhodospirillum rubrum. Biochim Biophys Acta. 1968; 161(1):162-9. DOI: 10.1016/0005-2787(68)90305-5. View

15.

Kurland C, Voynow P, Hardy S, Randall L, Lutter L . Physical and functional heterogeneity of E. coli ribosomes. Cold Spring Harb Symp Quant Biol. 1969; 34:17-24. DOI: 10.1101/sqb.1969.034.01.006. View

16.

Gillespie D, Spiegelman S . A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965; 12(3):829-42. DOI: 10.1016/s0022-2836(65)80331-x. View

17.

Salser W, Janin J, Levinthal C . Measurement of the unstable RNA in exponentially growing cultures of Bacillus subtilis and Escherichia coli. J Mol Biol. 1968; 31(2):237-66. DOI: 10.1016/0022-2836(68)90442-7. View

18.

Barnett W, Pennington Jr C, Fairfield S . Induction of euglena transfer RNA's by light. Proc Natl Acad Sci U S A. 1969; 63(4):1261-8. PMC: 223459. DOI: 10.1073/pnas.63.4.1261. View

19.

Gray E . Studies on the adaptive formation of photosynthetic structures in Rhodopseudomonas spheroides. I. Synthesis of macromolecules. Biochim Biophys Acta. 1967; 138(3):550-63. DOI: 10.1016/0005-2787(67)90551-5. View

20.

Traut R, Delius H, AHMAD-ZADEH C, Bickle T, Pearson P, TISSIERES A . Ribosomal proteins of E. Coli: stoichiometry and implications for ribosome structure. Cold Spring Harb Symp Quant Biol. 1969; 34:25-38. DOI: 10.1101/sqb.1969.034.01.007. View