Transcription of Polydeoxythymidylate Sequences in the Genome of the Cellular Slime Mold, Dictyostelium Discoideum

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 May 1

PMID 4525281

Citations 14

Authors

A Jacobson

R A Firtel

H Lodish

Affiliations

Soon will be listed here.

Abstract

Messenger RNA of the cellular slime mold, Dictyostelium discoideum, contains about equimolar amounts of two classes of poly(adenylic acid) [poly(A)]; one is about 25 nucleotides long and the second about 100 nucleotides long. At least half of the messenger RNA molecules contain one sequence each of poly(A)(25) and poly(A)(100); both poly(A) sequences are located near the 3' end of messenger RNA, and the kinetics of their appearance on messenger RNA precursor indicates that poly(A)(25) is added before poly(A)(100). Dictyostelium nuclear DNA contains 14,000-15,000 sequences of poly(dT)(25) which could code for the smaller poly(A) residues. The poly(A)(100) must be added post-transcriptionally. The poly(dT)(25) sequences are interspersed throughout the genome and may well represent transcription termination regions.

Citing Articles

A post-transcriptional regulon controlled by TtpA, the single tristetraprolin family member expressed in Dictyostelium discoideum.

Bai W, Wells M, Lai W, Hicks S, Burkholder A, Perera L Nucleic Acids Res. 2021; 49(20):11920-11937.

PMID: 34718768 PMC: 8599788. DOI: 10.1093/nar/gkab983.

Fluctuation in polyadenylate size and content in exponential- and stationary-phase cells of Saccharomyces cerevisiae.

Sogin S, Saunders C J Bacteriol. 1980; 144(1):74-81.

PMID: 6998972 PMC: 294591. DOI: 10.1128/jb.144.1.74-81.1980.

Repeating structure of cytoplasmic poly(A)-ribonucleoprotein.

Baer B, Kornberg R Proc Natl Acad Sci U S A. 1980; 77(4):1890-2.

PMID: 6929525 PMC: 348614. DOI: 10.1073/pnas.77.4.1890.

Sequence organization in Dictyostelium: unique structure at the 5'-ends of protein coding genes.

Kimmel A, Firtel R Nucleic Acids Res. 1983; 11(2):541-52.

PMID: 6828377 PMC: 325732. DOI: 10.1093/nar/11.2.541.

The binding of poly (rA) and poly (rU) to denatured DNA. II. Studies with natural DNAs.

Mol J, Borst P Nucleic Acids Res. 1976; 3(4):1029-51.

PMID: 1272801 PMC: 342963. DOI: 10.1093/nar/3.4.1029.

References

Riley M, Maling B . Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes. J Mol Biol. 1966; 20(2):359-89. DOI: 10.1016/0022-2836(66)90069-6. View

Bishop D, Claybrook J, Spiegelman S . Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967; 26(3):373-87. DOI: 10.1016/0022-2836(67)90310-5. View

Hayes F, LILLY E, RATLIFF R, Smith D, Williams D . Thermal transitions in mixtures of polydeoxyribodinucleotides. Biopolymers. 1970; 9(9):1105-17. DOI: 10.1002/bip.1970.360090911. View

Watts D, ASHWORTH J . Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture. Biochem J. 1970; 119(2):171-4. PMC: 1179339. DOI: 10.1042/bj1190171. View

Penman S, Rosbash M, Penman M . Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin. Proc Natl Acad Sci U S A. 1970; 67(4):1878-85. PMC: 283442. DOI: 10.1073/pnas.67.4.1878. View

Cocucci S, Sussman M . RNA in cytoplasmic and nuclear fractions of cellular slime mold amebas. J Cell Biol. 1970; 45(2):399-407. PMC: 2107906. DOI: 10.1083/jcb.45.2.399. View

Lebowitz P, Weissman S, Radding C . Nucleotide sequence of a ribonucleic acid transcribed in vitro from lambda phage deoxyribonucleic acid. J Biol Chem. 1971; 246(16):5120-39. View

Darnell J, Philipson L, Wall R, Adesnik M . Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971; 174(4008):507-10. DOI: 10.1126/science.174.4008.507. View

Philipson L, Wall R, GLICKMAN G, Darnell J . Addition of polyadenylate sequences to virus-specific RNA during adenovirus replication. Proc Natl Acad Sci U S A. 1971; 68(11):2806-9. PMC: 389530. DOI: 10.1073/pnas.68.11.2806. View

10.

Darnell J, Wall R, Tushinski R . An adenylic acid-rich sequence in messenger RNA of HeLa cells and its possible relationship to reiterated sites in DNA. Proc Natl Acad Sci U S A. 1971; 68(6):1321-5. PMC: 389181. DOI: 10.1073/pnas.68.6.1321. View

11.

Lee S, MENDECKI J, Brawerman G . A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971; 68(6):1331-5. PMC: 389183. DOI: 10.1073/pnas.68.6.1331. View

12.

Edmonds M, VAUGHAN Jr M, Nakazato H . Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971; 68(6):1336-40. PMC: 389184. DOI: 10.1073/pnas.68.6.1336. View

13.

Ling V . Fractionation and sequences of the large pyrimidine oligonucleotides from bacteriophage fd DNA. J Mol Biol. 1972; 64(1):87-102. DOI: 10.1016/0022-2836(72)90322-1. View

14.

Firtel R, Bonner J . Characterization of the genome of the cellular slime mold Dictyostelium discoideum. J Mol Biol. 1972; 66(3):339-61. DOI: 10.1016/0022-2836(72)90419-6. View

15.

Firtel R . Changes in the expression of single-copy DNA during development of the cellular slime mold Dictyostelium discoideum. J Mol Biol. 1972; 66(3):363-77. DOI: 10.1016/0022-2836(72)90420-2. View

16.

Molloy G, Sporn M, Kelley D, PERRY R . Localization of polyadenylic acid sequences in messenger ribonucleic acid of mammalian cells. Biochemistry. 1972; 11(17):3256-60. DOI: 10.1021/bi00767a020. View

17.

Pieczenik G, Barrell B, Gefter M . Bacteriophage phi 80-induced low molecular weight RNA. Arch Biochem Biophys. 1972; 152(1):152-65. DOI: 10.1016/0003-9861(72)90203-2. View

18.

Firtel R, Jacobson A, Lodish H . Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum. Nat New Biol. 1972; 239(95):225-8. DOI: 10.1038/newbio239225a0. View

19.

Adesnik M, Salditt M, Thomas W, Darnell J . Evidence that all messenger RNA molecules (except histone messenger RNA) contain Poly (A) sequences and that the Poly(A) has a nuclear function. J Mol Biol. 1972; 71(1):21-30. DOI: 10.1016/0022-2836(72)90397-x. View

20.

Stoltzfus C, Banerjee A . Two oligonucleotide classes of single-stranded ribopolymers in reovirus A-rich RNA. Arch Biochem Biophys. 1972; 152(2):733-43. DOI: 10.1016/0003-9861(72)90269-x. View