Glycopeptides from Surface Membranes of Neuroblastoma Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1973 Jun 1

PMID 4515927

Citations 18

Authors

M C GLICK

Y KIMHI

U Z LITTAUER

Affiliations

Soon will be listed here.

Abstract

Sequential removal of surface glycopeptides was achieved by subjection of mouse neuroblastoma cells to a two-step trypsin treatment under different conditions. The glycopetides released by each trypsinization step were digested by Pronase and examined on columns of Sephadex G-50. Different chromatographic patterns were found for the two digests. Thus, several groups of glycopeptides can be distinguished by the trypsinization procedure. One group is readily removed and appears to be at a more accessible location on the cell surface. Among the four neuroblastoma clones examined, the glycopeptide patterns from axon-forming cells differed from those of axon-minus cells.

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