Purification of Oncornaviruses by Agglutination with Concanacalin A (murine Leukemia Virus-phytohemagglutinin-friend Virus)

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1973 May 1

PMID 4514301

Citations 11

Authors

M L Stewart

D F Summers

R Soeiro

B N Fields

J V Maizel Jr

Affiliations

Soon will be listed here.

Abstract

Concanavalin A (Con A) has been used to rapidly and selectively agglutinate murine and avian oncornavirions from culture medium or plasma. The agglutinated virus was concentrated rapidly and gently by low-speed centrifugation and solubilization with alpha-methyl mannoside. Infectious virus was purified 2.3 times with respect to nucleic-acid content, and more than 60% of its infectivity was recovered. Infectious particles of densities 1.18 and 1.16 g/cm(3) were found in mouse cells infected with Friend virus. Con A reacted only with particles of density 1.16 g/cm(3), indicating heterogeneity with respect to carbohydrate content or structure as well as buoyant density. Electron microscopy of virus agglutinated with Con A showed a zone of Con A-glycoprotein complexes averaging 12-15 nm in thickness.

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