Cytoplasmic Adenylylation and Processing of Maternal RNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1973 Feb 1

PMID 4510284

Citations 21

Authors

I Slater

D Gillespie

D W Slater

Affiliations

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Abstract

Molecular hybridization between [(3)H]-poly(U) and unlabeled RNA prepared from sea urchin eggs and embryos has been used to contrast the subcellular localization as well as the size distribution of adenylylated maternal RNA preexisting in the unfertilized egg with that adenylylated as a function of fertilization. Evidence reported establishes that such preadenylylated genetic messages are predominantly located in the ovum's subribosomal fraction and that fertilization elicits a rapid reallocation of these latent transcripts into the zygote's ribosomal fraction. Examination of the size distribution of the adenylylated RNA further demonstrates that the unfertilized egg contains a substantial population of RNA transcripts of exceptionally high molecular weight that are used as primers for the 2-fold net synthesis of poly(A) that follows fertilization. The poly(A)-rich tracts are shown to be covalently bonded to RNA. Assessment of the poly(A) content of nuclear and cytoplasmic fractions suggests that the function of poly(A) is not confined to the transport of genetic messages from the nucleus.

Citing Articles

[Ribonucleoprotein-particles from telotrophic meroistic ovary ofDysdercus intermedius Dist. (Heteroptera, Pyrrhoc.) and their behaviour in a cell-free protein synthesizing system].

Winter H Wilhelm Roux Arch Entwickl Mech Org. 2017; 175(2):103-127.

PMID: 28304716 DOI: 10.1007/BF00574296.

Pattern of ribonucleic acid synthesis during germination of Allomyces macrogynus mitospores.

Smith B, Burke D J Bacteriol. 1980; 143(3):1353-61.

PMID: 7190969 PMC: 294511. DOI: 10.1128/jb.143.3.1353-1361.1980.

Poly(adenylic acid) in small amounts, free or covalently linked to substrate, protects RNA from hydrolysis by ribonuclease.

Karpetsky T, Shriver K, Levy C Biochem J. 1981; 193(1):311-24.

PMID: 6171250 PMC: 1162604. DOI: 10.1042/bj1930311.

Possible involvement of poly(A) in protein synthesis.

Jacobson A, Favreau M Nucleic Acids Res. 1983; 11(18):6353-68.

PMID: 6137807 PMC: 326378. DOI: 10.1093/nar/11.18.6353.

Cell-free translation of maternal messenger RNA from sea urchin eggs.

Gross K, Jacobs-Lorena M, Baglioni C, Gross P Proc Natl Acad Sci U S A. 1973; 70(9):2614-8.

PMID: 4582192 PMC: 427067. DOI: 10.1073/pnas.70.9.2614.

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