31P NMR Studies of the Solution Structure and Dynamics of Nucleosomes and DNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1979 Apr 1

PMID 450708

Citations 9

Authors

L Klevan

I M Armitage

D M Crothers

Affiliations

Soon will be listed here.

Abstract

31P NMR studies of 140 base pair DNA fragments in nucleosomes and free in solution show no detectable change in the internucleotide 31P chemical shift or linewidth when DNA is packaged into nucleosomes. Measurements of 31P spin-lattice relaxation times T1 and 31P-[H] nuclear Overhauser enhancements revealed internal motion with a correlation time of about 4 x 10(-10) sec in double helical DNA, both free in solution and bound to nucleosomal core proteins. This result implies greater dynamic mobility in double helical DNA than has previously been supposed.

Citing Articles

The effect of ethidium bromide on DNA internal motions.

Hogan M, Jardetzky O Biophys J. 2009; 32(1):632-3.

PMID: 19431402 PMC: 1327367. DOI: 10.1016/S0006-3495(80)85000-4.

Conformation of 145 base pair length poly (dG-dC) . poly (dG-dC) in solution and in association with histones.

Simpson R, Shindo H Nucleic Acids Res. 1980; 8(9):2093-103.

PMID: 7433135 PMC: 324061. DOI: 10.1093/nar/8.9.2093.

13C NMR relaxation and conformational flexibility of the deoxyribose ring.

Levy G, Craik D, Chou Y, London R Nucleic Acids Res. 1982; 10(19):6067-83.

PMID: 7145716 PMC: 320951. DOI: 10.1093/nar/10.19.6067.

DNA motions in the nucleosome core particle.

Wang J, Hogan M, Austin R Proc Natl Acad Sci U S A. 1982; 79(19):5896-900.

PMID: 6964394 PMC: 347017. DOI: 10.1073/pnas.79.19.5896.

Molecular motion of DNA as measured by triplet anisotropy decay.

Hogan M, Wang J, Austin R, Monitto C, Hershkowitz S Proc Natl Acad Sci U S A. 1982; 79(11):3518-22.

PMID: 6954497 PMC: 346452. DOI: 10.1073/pnas.79.11.3518.

References

Rich A, Davies D, Crick F, Watson J . The molecular structure of polyadenylic acid. J Mol Biol. 1961; 3:71-86. DOI: 10.1016/s0022-2836(61)80009-0. View

Cotter R, Lilley D . The conformation of DNA and protein within chromatin subunits. FEBS Lett. 1977; 82(1):63-8. DOI: 10.1016/0014-5793(77)80886-7. View

Kallenbach N, Appleby D, Bradley C . 31P magnetic resonance of DNA in nucleosome core particles of chromatin. Nature. 1978; 272(5649):134-8. DOI: 10.1038/272134a0. View

Cozzone P, Jardetzky O . Phosphorus-31 Fourier transform nuclear magnetic resonance study of mononucleotides and dinucleotides. 2. Coupling constants. Biochemistry. 1976; 15(22):4860-5. DOI: 10.1021/bi00667a017. View

Cozzone P, Jardetzky O . Phosphorus-31 Fourier transform nuclear magnetic resonance study of mononucleotides and dinucleotides. 1. Chemical shifts. Biochemistry. 1976; 15(22):4853-9. DOI: 10.1021/bi00667a016. View

Klevan L, Hogan M, Dattagupta N, Crothers D . Electric dichroism studies of the size and shape of nucleosomal particles. Cold Spring Harb Symp Quant Biol. 1978; 42 Pt 1:207-14. DOI: 10.1101/sqb.1978.042.01.023. View

Grosjean H, de Henau S, Crothers D . On the physical basis for ambiguity in genetic coding interactions. Proc Natl Acad Sci U S A. 1978; 75(2):610-4. PMC: 411305. DOI: 10.1073/pnas.75.2.610. View

Hogan M, Dattagupta N, Crothers D . Transient electric dichroism of rod-like DNA molecules. Proc Natl Acad Sci U S A. 1978; 75(1):195-9. PMC: 411212. DOI: 10.1073/pnas.75.1.195. View

Klevan L, Crothers D . Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin. Nucleic Acids Res. 1977; 4(12):4077-89. PMC: 343227. DOI: 10.1093/nar/4.12.4077. View

10.

Finch J, Lutter L, Rhodes D, Brown R, Rushton B, Levitt M . Structure of nucleosome core particles of chromatin. Nature. 1977; 269(5623):29-36. DOI: 10.1038/269029a0. View

11.

Barry C, North A, Glasel J, Williams R, Xavier A . Quantitative determination of mononucleotide conformations in solution using lanthanide ion shift and broadenine NMR probes. Nature. 1971; 232(5308):236-45. DOI: 10.1038/232236a0. View

12.

Akasaka K . Proton and phosphorus magnetic relaxation studies on the dynamic structure of single-stranded polyriboadenylic acid. Biopolymers. 1974; 13(11):2273-80. DOI: 10.1002/bip.1974.360131109. View

13.

Barry C, Glasel J, Williams R, Xavier A . Quantitative determination of conformations of flexible molecules in solution using lanthanide ions as nuclear magnetic resonance probes: application to adenosine-5'-monophosphate. J Mol Biol. 1974; 84(4):471-09. DOI: 10.1016/0022-2836(74)90110-7. View

14.

Gueron M, Shulman R . 31P magnetic resonance of tRNA. Proc Natl Acad Sci U S A. 1975; 72(9):3482-5. PMC: 433018. DOI: 10.1073/pnas.72.9.3482. View

15.

Kondo N, Danyluk S . Conformational properties of adenylyl-3' leads to 5'-adenosine in aqueous solution. Biochemistry. 1976; 15(4):756-68. DOI: 10.1021/bi00649a006. View

16.

Patel D . Proton and phosphorus NMR studies of d-CpG(pCpG)n duplexes in solution. Helix-coil transition and complex formation with actinomycin-D. Biopolymers. 1976; 15(3):533-58. DOI: 10.1002/bip.1976.360150310. View

17.

Gorenstein D, Findlay J, Momii R, Luxon B, Kar D . Temperature dependence of the 31P chemical shifts of nucleic acids. A prode of phosphate ester torsional conformations. Biochemistry. 1976; 15(17):3796-803. DOI: 10.1021/bi00662a023. View

18.

Patel D, Canuel L . Nuclear magnetic resonance studies of the helix-coil transition of poly (dA-dT) in aqueous solution. Proc Natl Acad Sci U S A. 1976; 73(3):674-8. PMC: 335980. DOI: 10.1073/pnas.73.3.674. View

19.

Gorenstein D, Kar D . 31-P chemical shifts in phosphate diester monoanions. Bond angle and torsional angle effects. Biochem Biophys Res Commun. 1975; 65(3):1073-80. DOI: 10.1016/s0006-291x(75)80495-5. View

20.

Crick F, Klug A . Kinky helix. Nature. 1975; 255(5509):530-3. DOI: 10.1038/255530a0. View