Mitochondrial DNA Polymerase, Deoxyribonuclease and Ribonuclease H Activities from Brain of Chick Embryo

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1974 Sep 1

PMID 4476911

Citations 1

Authors

L Soriano

J Smith

Y CROISILLE

B Dastugue

Affiliations

Soon will be listed here.

Abstract

R-DNA polymerase, D-DNA polymerase, DNase and RNase H activities in mitochondria from chick embryonic brain were studied by ion-exchange chromatography. Two main fractions were separated according to their chromatographic behaviour: a fraction M Ib which is eluted with the washing buffer from two successive DEAE-cellulose columns and a fraction M IV which is eluted at 400 mM KC1 from a phosphocellulose column. Although the two fractions contain both the DNA polymerase and the degrading activities, all the specific activities are higher in fraction M IV than in fraction M Ib. Heat inactivation experiments have shown that R-DNA polymerase is inactivated in both fractions, whereas RNase H and DNase are not affected. Thus, degrading activities and R-DNA polymerase activity seem to be catalyzed by different molecular entities. However the fact that in most cases these activities co-chromatograph suggests that the corresponding molecules form rather stable complexes.

Citing Articles

Endogenous DNA-directed DNA synthesising system in a microsomal fraction of embryonic chick brain.

Smith J, Soriano L Nucleic Acids Res. 1977; 4(3):641-8.

PMID: 866184 PMC: 342468. DOI: 10.1093/nar/4.3.641.

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