Characterization of Ribonucleic Acids from the Venom Glands of Crotalus Durissus Terrifucus (Ophidia, Reptilia) After Manual Extraction of the Venom. Studies on Template Activity and Base Composition

Overview

Journal Biochem J

Specialty Biochemistry

Date 1974 Apr 1

PMID 4463939

Citations 1

Authors

F L De Lucca

M T Imaizumi

A Haddad

Affiliations

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Abstract

RNA synthesis in the venom glands of Crotalus durissus terrificus was stimulated by the manual extraction of the venom (milking). RNA was extracted from venom glands activated by milking and fractionated by centrifugation through sucrose density gradients. Template activity for protein synthesis and base composition of the RNA fractions were studied. RNA fractions that sediment between 18S and 4S had the highest template activity. The base composition analysis indicated that the 28S and 18S rRNA have a C+G content of 65.4 and 58% respectively. The ;melting' temperature (T(m)) of DNA in 0.15m-NaCl-0.015m-trisodium citrate, pH7.0, was 85 degrees C, corresponding to a C+G content of 38%. The base ratio of the RNA fractions that showed a high template activity was intermediate between that of rRNA and homologous DNA. The possible role of these fractions in the synthesis of the two main toxins (crotoxin and crotamine) of the South American rattlesnake's venom is discussed.

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PMID: 2124275 DOI: 10.1007/BF02101122.

References

Singer M, Leder P . Messenger RNA: an evaluation. Annu Rev Biochem. 1966; 35:195-230. DOI: 10.1146/annurev.bi.35.070166.001211. View

FRAENKEL-CONRAT H, Singer B . Fractionation and composition of crotoxin. Arch Biochem Biophys. 1956; 60(1):64-73. DOI: 10.1016/0003-9861(56)90397-6. View

Darnell Jr J . Ribonucleic acids from animal cells. Bacteriol Rev. 1968; 32(3):262-90. PMC: 408298. DOI: 10.1128/br.32.3.262-290.1968. View

Lim L, Canellakis E . Adenine-rich polymer associated with rabbit reticulocyte messenger RNA. Nature. 1970; 227(5259):710-2. DOI: 10.1038/227710a0. View

Stavnezer J, Huang R . Synthesis of a mouse immunoglobulin light chain in a rabbit reticulocyte cell-free system. Nat New Biol. 1971; 230(14):172-6. DOI: 10.1038/newbio230172a0. View

Mathews M, Osborn M, Lingrel J . Translation of globin messenger RNA in a heterologous cell-free system. Nature. 1971; 233(5320):206-9. View

Burr H, Lingrel J . Poly A sequences at the 3' termini of rabbit globin mRNAs. Nat New Biol. 1971; 233(36):41-3. DOI: 10.1038/newbio233041a0. View

Rotenberg D, Bamberger E, Kochva E . Studies on ribonucleic acid synthesis in the venom glands of Vipera palaestinae (Ophidia, Reptilia). Biochem J. 1971; 121(4):609-12. PMC: 1176636. DOI: 10.1042/bj1210609. View

Mathews M, Osborn M, Berns A, Bloemendal H . Translation of two messenger RNAs from lens in a cell free system from Krebs II ascites cells. Nat New Biol. 1972; 236(61):5-7. DOI: 10.1038/newbio236005a0. View

10.

Berns A, Strous G, Bloemendal H . Heterologous in vitro synthesis of lens -crystallin polypeptide. Nat New Biol. 1972; 236(61):7-9. DOI: 10.1038/newbio236007a0. View

11.

Oron U, BDOLAH A . Regulation of protein synthesis in the venom gland of viperid snakes. J Cell Biol. 1973; 56(1):177-90. PMC: 2108839. DOI: 10.1083/jcb.56.1.177. View

12.

De Lucca F, Imaizumi M . Synthesis of ribonucleic acid in the venom gland of Crotalus durissus terrificus (Ophidia, Reptilia) after manual extraction of tne venom. Biochem J. 1972; 130(2):335-42. PMC: 1174412. DOI: 10.1042/bj1300335. View

13.

Hunt J . Terminal sequence studies of high-molecular-weight ribonucleic acid. The 3' termini of rabbit globin messenger ribonucleic acid. Biochem J. 1973; 131(2):315-25. PMC: 1177471. DOI: 10.1042/bj1310315. View

14.

Reed J, Wintersberger E . Adenylic acid-rich sequences in messenger RNA from yeast polysomes. FEBS Lett. 1973; 32(2):213-7. DOI: 10.1016/0014-5793(73)80835-x. View

15.

WARSHAWSKY H, Haddad A, Goncalves R, VALERI V, De Lucca F . Fine structure of the venom gland epithelium of the South American rattlesnake and radioautographic studies of protein formation by the secretory cells. Am J Anat. 1973; 138(1):79-119. DOI: 10.1002/aja.1001380106. View

16.

Giorgini J, De Lucca F . Thermolability of 28S ribosomal ribonucleic acid from the liver of Crotalus durissus terrificus (Ophidia, Reptilia). Biochem J. 1973; 135(1):73-9. PMC: 1165790. DOI: 10.1042/bj1350073. View

17.

Greenbaum A, Slater T . Studies on the particulate components of rat mammary gland. II. Changes in the levels of the nucleic acids of the mammary glands of rats during pregnancy, lactation and mammary involution. Biochem J. 1957; 66(1):155-61. PMC: 1199981. DOI: 10.1042/bj0660155. View

18.

Kitazume Y, Ycas M, VINCENT W . Metabolic properties of a ribonucleic acid fraction in yeast. Proc Natl Acad Sci U S A. 1962; 48:265-82. PMC: 220768. DOI: 10.1073/pnas.48.2.265. View

19.

Katz S, COMB D . A NEW METHOD FOR THE DETERMINATION OF THE BASE COMPOSITION OF RIBONUCLEIC ACID. J Biol Chem. 1963; 238:3065-7. View

20.

Kitazume Y, Ycas M . THE CALCULATED COMPOSITION OF NEWLY SYNTHESIZED YEAST RIBONUCLEIC ACID. Biochim Biophys Acta. 1963; 76:391-400. View