Genetic Control of Chlorophyll Biosynthesis in Chlamydomonas. Analysis of Mutants at Two Loci Mediating the Conversion of Protoporphyrin-IX to Magnesium Protoporphyrin

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1974 Dec 1

PMID 4436384

Citations 18

Authors

W Y Wang

W L Wang

J E Boynton

N W Gillham

Affiliations

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Abstract

In this report we describe two nonallelic Mendelian protoporphyrin accumulating mutants br(s)-1 and br(c)-1. Results of experiments with these mutants lead us to postulate that porphyrin biosynthesis branches into light and dark steps between protoporphyrin-IX and magnesium protoporphyrin. We hypothesize that the br(c) locus controls a dark step while the br(s) locus either controls a step in the main pathway before the branch or mediates the preparation of the magnesium ion for its insertion into protoporphyrin-IX. The br(s)-1 mutant is thought to be light sensitive because a block prior to the branch point in the porphyrin pathway prevents chlorophyll formation in either the light or the dark. The br(c)-1 mutant, which also accumulates protoporphyrin in the dark, forms chlorophyll and chloroplast lamellae when transferred to the light, showing that function of the porphyrin pathway is normal in the light.

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