Posttranscriptional Control of Tyrosine Aminotransferase Synthesis by Insulin

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1970 Jun 1

PMID 4393739

Citations 12

Authors

T D Gelehrter

G M Tomkins

Affiliations

Soon will be listed here.

Abstract

Adrenal steroid hormones induce the synthesis of tyrosine amino-transferase (TAT) in HTC cells, an established line of rat hepatoma cells in tissue culture. The addition of insulin to cells previously induced in a serum-free medium results in a rapid two- to threefold further increase in the rate of synthesis of TAT and a small increase in total amino acid incorporation. These changes do not require concomitant RNA synthesis, suggesting that insulin acts at a step in protein synthesis beyond that of gene transcription. Although the effects of insulin on HTC cells are similar to those caused by dialyzed bovine serum, evidence is presented that insulin and serum affect different aspects of TAT synthesis. Unlike the glucocorticoids, insulin does not cause the accumulation of TAT mRNA, nor the sustained induction of TAT. The continued presence of the inducing steroid is required to permit maximal expression of the insulin effect.

Citing Articles

Elevated serum levels of cysteine and tyrosine: early biomarkers in asymptomatic adults at increased risk of developing metabolic syndrome.

Mohorko N, Petelin A, Jurdana M, Biolo G, Jenko-Praznikar Z Biomed Res Int. 2015; 2015:418681.

PMID: 25821801 PMC: 4364114. DOI: 10.1155/2015/418681.

TATN-1 mutations reveal a novel role for tyrosine as a metabolic signal that influences developmental decisions and longevity in Caenorhabditis elegans.

Ferguson A, Roy S, Kormanik K, Kim Y, Dumas K, Ritov V PLoS Genet. 2014; 9(12):e1004020.

PMID: 24385923 PMC: 3868569. DOI: 10.1371/journal.pgen.1004020.

Cultured hepatoma cells for the study of enzyme regulation: induction of ornithine decarboxylase by insulin and asparagine.

POTTER V, Evanson T, Gayda D, Gurr J In Vitro. 1984; 20(9):723-31.

PMID: 6389319 DOI: 10.1007/BF02618878.

Time course study of L-tyrosine aminotransferase induction in rat liver cell lines.

Chessebeuf M, Fischbach M, PADIEU P Cell Biol Toxicol. 1984; 1(1):31-40.

PMID: 6152899 DOI: 10.1007/BF00125563.

Relationship of phenotypic expression of blood group H to changes in growth kinetics of cultured primary and transformed epithelioid cells.

Kuhns W, Pann C Am J Pathol. 1973; 73(3):789-806.

PMID: 4588184 PMC: 1904098.

References

Paul J, Pearson E . The action of insulin on the metabolism of cell cultures. J Endocrinol. 1960; 21:287-94. DOI: 10.1677/joe.0.0210287. View

Martin Jr D, Tomkins G, Bresler M . Control of specific gene expression examined in synchronized mammalian cells. Proc Natl Acad Sci U S A. 1969; 63(3):842-9. PMC: 223529. DOI: 10.1073/pnas.63.3.842. View

Temin H . Studies on carcinogenesis by avian sarcoma viruses. VI. Differential multiplication of uninfected and of converted cells in response to insulin. J Cell Physiol. 1967; 69(3):377-84. DOI: 10.1002/jcp.1040690314. View

Leslie I, FULTON W, Sinclair R . The metabolism of human embryonic and malignant cells and their response to insulin. Biochim Biophys Acta. 1957; 24(2):365-80. DOI: 10.1016/0006-3002(57)90207-x. View

Hager C, Kenney F . Regulation of tyrosine-alpha-ketoglutarate transaminase in rat liver. VII. Hormonal effects of synthesis in the isolated, perfused liver. J Biol Chem. 1968; 243(12):3296-300. View

Lieberman I, Ove P . Growth factors for mammalian cells in culture. J Biol Chem. 1959; 234:2754-8. View

Granner D, CHASE L, Aurbach G, Tomkins G . Tyrosine aminotransferase: enzyme induction independent of adenosine 3', 5'-monophosphate. Science. 1968; 162(3857):1018-20. DOI: 10.1126/science.162.3857.1018. View

Madison L . Role of insulin in the hepatic handling of glucose. Arch Intern Med. 1969; 123(3):284-92. View

Holten D, Kenney F . Regulation of tyrosine alpha-ketoglutarate transaminase in rat liver. VI. Induction by pancreatic hormones. J Biol Chem. 1967; 242(19):4372-7. View

10.

Tomkins G, Gelehrter T, Granner D, Martin Jr D, Samuels H, Thompson E . Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA. Science. 1969; 166(3912):1474-80. DOI: 10.1126/science.166.3912.1474. View

11.

Chambers J, Georg R, Bass A . Effect of hydrocortisone and insulin on uptake of alpha-aminoisobutyric acid by isolated perfused rat liver. Mol Pharmacol. 1965; 1(1):66-76. View

12.

Thompson E, Tomkins G, Curran J . Induction of tyrosine alpha-ketoglutarate transaminase by steroid hormones in a newly established tissue culture cell line. Proc Natl Acad Sci U S A. 1966; 56(1):296-303. PMC: 285710. DOI: 10.1073/pnas.56.1.296. View

13.

Auricchio F, Martin Jr D, Tompkins G . Control of degradation and synthesis of induced tyrosine aminotransferase studied in hepatoma cells in culture. Nature. 1969; 224(5221):806-8. DOI: 10.1038/224806b0. View

14.

Peterkofsky B, Tomkins G . Evidence for the steroid-induced accumulation of tyrosine-aminotransferase messenger RNA in the absence of protein synthesis. Proc Natl Acad Sci U S A. 1968; 60(1):222-8. PMC: 539106. DOI: 10.1073/pnas.60.1.222. View

15.

Gelehrter T, Tomkins G . Control of tyrosine aminotransferase synthesis in tissue culture by a factor in serum. Proc Natl Acad Sci U S A. 1969; 64(2):723-30. PMC: 223404. DOI: 10.1073/pnas.64.2.723. View

16.

Gelehrter T, Tomkins G . The role of RNA in the hormonal induction of tyrosine aminotransferase in mammalian cells in tissue culture. J Mol Biol. 1967; 29(1):59-76. DOI: 10.1016/0022-2836(67)90181-7. View

17.

Lockwood D, Voytovich A, STOCKDALE F, TOPPER Y . Insulin-dependent DNA polymerase and DNA synthesis in mammary epithelial cells in vitro. Proc Natl Acad Sci U S A. 1967; 58(2):658-64. PMC: 335685. DOI: 10.1073/pnas.58.2.658. View

18.

WICKS W . Induction of tyrosine-alpha-ketoglutarate transaminase in fetal rat liver. J Biol Chem. 1968; 243(5):900-6. View

19.

Schwartz A, Amos H . Insulin dependence of cells in primary culture: influence on ribo-some integrity. Nature. 1968; 219(5161):1366-7. DOI: 10.1038/2191366a0. View