A Survey of Some Active Sheep Pineal Fractions and a Discussion on the Possible Significance of Pteridines in Those Fractions in in Vitro and in Vivo Assays

Overview

Journal J Neural Transm

Specialties Neurology
Physiology

Date 1979 Jan 1

PMID 438806

Citations 1

Authors

I EBELS

A de Moree

A Hus-Citharel

A MOSZKOWSKA

Affiliations

Soon will be listed here.

Abstract

Aqueous extracts of sheep pineals are separated on Sephadex G-25 fraction with antigonadotropic activity are ultrafiltered through the membrane UM 2. The UM 2 filtrate is subsequently filtered through the membrane UM 05. The actions of the different fractions on the anterior hypophysis and hypothalamus are discussed. The fractions which show an activity are further separated on Sephadex G-10. The active Sephadex G-10 fractions of the UM 05 filtrate acting on the anterior hypophysis in vitro are purified by electrophoresis and paper chromatography. After elution of different fluorescence bands the main inhibitory activity is found in a region, with the same Rf value as synthetic 6-biopterin. Gas liquid chromatography and mass spectrometry studies of the isolated fractions are carried out. The mass spectrum of an isolated compound from that fraction is identical with that of synthetic 6-biopterin. Thinlayer chromatography and the results of the Crithidia fasciculata test reveal that the isolated compound is probably identical with 6-L-erythrobiopterin. The activity of the active paper chromatography fraction (C4) is compared with the activity of three synthetic biopterin-preparations in vitro and in vivo.

Citing Articles

Pteridines as nonretinal regulators of light-dependent melatonin biosynthesis.

CREMER-BARTELS G, EBELS I Proc Natl Acad Sci U S A. 1980; 77(5):2415-8.

PMID: 6930641 PMC: 349409. DOI: 10.1073/pnas.77.5.2415.

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