Distribution of Beta-adrenergic Receptors on Human Lymphocyte Subpopulations

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1979 Dec 1

PMID 43789

Citations 13

Authors

R Pochet

G Delespesse

P W Gausset

H Collet

Affiliations

Soon will be listed here.

Abstract

A technique is described allowing the quantification and the characterization of specific beta-adrenergic receptors in intact living human lymphocytes. 125I-Iodohydroxybenzylpindolol, a potent beta-adrenergic antagonist was used to label specific binding sites on unfractionated lymphoid cells and on purified subpopulations of T (F1 and F2) and B cells. F1 and F2 were obtained by filtration through nylon wool column as previously described (Delespesse et al., 1976), they differ in their response to mitogens, and in their interactions with adherent cells and B cells. 125I-HYP binding to unfractionated lymphocytes was a saturable, stereospecific and rapid process with a dissociation constant of 2.5 10(-10) M and a binding capacity of 400--600 sites/cell. Bindings on unfractionated lymphocytes, purified B cells and T cells of the F2 fraction were similar. No detectable binding was noted on T cells from the F1 fraction. Enriched T cells obtained by a rosetting technique displayed 200 receptors/cell.

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References

Delespesse G, Duchateau J, Gausset P, Govaerts A . In vitro response of subpopulations of human tonsil lymphocytes. I. Cellular collaboration in the proliferative response to PHA and Con A. J Immunol. 1976; 116(2):437-45. View

Brown E, Gardner J, Aurbach G . Direct determination of ligand interactions with beta-adrenergic receptors on intact turkey erythrocytes: correlation of binding with biological activity. Endocrinology. 1976; 99(5):1370-6. DOI: 10.1210/endo-99-5-1370. View

Williams L, Snyderman R, Lefkowitz R . Identification of beta-adrenergic receptors in human lymphocytes by (-) (3H) alprenolol binding. J Clin Invest. 1976; 57(1):149-55. PMC: 436634. DOI: 10.1172/JCI108254. View

Schmitt H, Pochet R . In vivo labelling of beta-adrenergic receptors on rat glioma cells. FEBS Lett. 1977; 76(2):302-5. DOI: 10.1016/0014-5793(77)80173-7. View

Atkinson J, Wedner H, Parker C . Two novel stimuli of cyclic adenosine 3',5'-monophosphate (cAMP) in human lymphocytes. J Immunol. 1975; 115(4):1023-7. View

Wolfe B, Harden T, Molinoff P . In vitro study of beta-adrenergic receptors. Annu Rev Pharmacol Toxicol. 1977; 17:575-604. DOI: 10.1146/annurev.pa.17.040177.003043. View

Niaudet P, Beaurain G, Bach M . Differences in effect of isoproterenol stimulation on levels of cyclic AMP in human B and T lymphocytes. Eur J Immunol. 1976; 6(11):834-6. DOI: 10.1002/eji.1830061117. View

Roszkowski W, Plaut M, Lichtenstein L . Selective display of histamine receptors on lymphocytes. Science. 1977; 195(4279):683-5. DOI: 10.1126/science.190677. View

Ross E, Maguire M, Sturgill T, Biltonen R, GILMAN A . Relationship between the beta-adrenergic receptor and adenylate cyclase. J Biol Chem. 1977; 252(16):5761-75. View

10.

Lefkowitz R . Identification and regulation of alpha- and beta-adrenergic receptors. Fed Proc. 1978; 37(2):123-9. View

11.

Guellaen G, Vauquelin G, Strosberg D, Hanoune J . Characterization with [3H] dihydroergocryptine of the alpha-adrenergic receptor of the hepatic plasma membrane. Comparison with the beta-adrenergic receptor in normal and adrenalectomized rats. J Biol Chem. 1978; 253(4):1114-20. View

12.

Terasaki W, Brooker G . [125I]Iodohydroxybenzylpindolol binding sites on intact rat glioma cells. Evidence for beta-adrenergic receptors of high coupling efficiency. J Biol Chem. 1978; 253(15):5418-25. View

13.

Insel P, Stoolman L . Radioligand binding to beta adrenergic receptors of intact cultured S49 cells. Mol Pharmacol. 1978; 14(4):549-61. View

14.

Galant S, Underwood S, Lundak T, Groncy C, Mouratides D . Heterogeneity of human lymphocyte subpopulations to pharmacologic stimulation. I. Lymphocyte responsiveness to beta adrenergic agents. J Allergy Clin Immunol. 1978; 62(6):349-56. DOI: 10.1016/0091-6749(78)90135-5. View

15.

Pochet R, Schmitt H . Re-evaluation of the number of specific beta-adrenergic receptors on muscle cells. Nature. 1979; 277(5691):58-60. DOI: 10.1038/277058a0. View

16.

Jondal M, Holm G, Wigzell H . Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2):207-15. PMC: 2139198. DOI: 10.1084/jem.136.2.207. View

17.

PreudHomme J, Flandrin G . Identification by peroxidase staining of monocytes in surface immunofluorescence tests. J Immunol. 1974; 113(5):1650-3. View

18.

Henney C, Bourne H, Lichtenstein L . The role of cyclic 3',5' adenosine monophosphate in the specific cytolytic activity of lymphocytes. J Immunol. 1972; 108(6):1526-34. View

19.

Melmon K, Bourne H, Weinstein Y, Shearer G, Kram J, BAUMINGER S . Hemolytic plaque formation by leukocytes in vitro. Control by vasoactive hormones. J Clin Invest. 1974; 53(1):13-21. PMC: 301433. DOI: 10.1172/JCI107530. View