The Binding of Rabbit IgG and Its Enzymatically Derived Fragments to Homologous Peritoneal Macrophages

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1979 Mar 1

PMID 437840

Citations 5

Authors

M Ganczakowski

R G Leslie

Affiliations

Soon will be listed here.

Abstract

Rabbit IgG and its Fab, Fc and pFc' fragments, prepared by papain or peptic digestion, were assayed for binding to homologous peritoneal macrophages. The binding affinity of IgG for the peritoneal macrophages (Ka = 5.9 +/- 1.6 x 10(5) L/M) was comparable to that recorded with alveolar macrophages (7.6 +/- 1.8 x 10(5) L/M, Arend & Mannik, 1973) but the number of receptor sites per peritoneal cell (4.6 +/- 2.1 x10(6)) was about four-fold greater than on the latter. Of the fragments, only Fc bound to macrophages with an affinity comparable to intact IgG; pFc' bound weakly and Fab was totally inactive. These data, taken with a recent study involving rabbit IgG and guinea-pig macrophages (Ovary, Saluk, Quijada & Lamm, 1976), indicate that the primary IgG binding site for macrophages is located in the C gamma 2 domain.

Citing Articles

The localization of the binding site(s) on human IgG1 for the Fc receptors on homologous monocytes and heterologous mouse macrophages.

Ratcliffe A, STANWORTH D Immunology. 1983; 50(1):93-100.

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Quantitative studies of Fc receptors on human monocytes: characterization by binding of homologous and heterologous monomeric IgG and soluble immune complexes of different composition.

Rasmussen J, Brandslund I, Leslie R, Svehag S Immunology. 1983; 49(3):537-44.

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The expression of Fc receptors on guinea-pig peritoneal macrophages and neutrophils.

Coupland K, Leslie R Immunology. 1983; 48(4):647-56.

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Fc receptor--more answers, more questions.

Fornusek L, Vetvicka V Folia Microbiol (Praha). 1984; 29(6):476-516.

PMID: 6396178 DOI: 10.1007/BF02873160.

Species specificity in the binding of IgG to macrophages.

Leslie R, Niemetz A Immunology. 1979; 37(4):835-40.

PMID: 500129 PMC: 1457146.

References

Porter R . The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959; 73:119-26. PMC: 1197021. DOI: 10.1042/bj0730119. View

Mandy W, RIVERS M, Nisonoff A . Recombination of univalent subunits derived from rabbit antibody. J Biol Chem. 1961; 236:3221-6. View

MELAMED M, Green N . AVIDIN. 2. PURIFICATION AND COMPOSITION. Biochem J. 1963; 89:591-9. PMC: 1202467. DOI: 10.1042/bj0890591. View

Alexander M, Andrews J, Leslie R, Wood N . The binding of human and guinea-pig IgG subclasses to homologous macrophage and monocyte Fc receptors. Immunology. 1978; 35(1):115-23. PMC: 1457241. View

Alexander M, Leslie R, Cohen S . Cytophilic activity of enzymatically derived fragments of guinea pig IgG2. Eur J Immunol. 1976; 6(2):101-7. DOI: 10.1002/eji.1830060206. View

Huber R, Deisenhofer J, Colman P, Matsushima M, PALM W . Crystallographic structure studies of an IgG molecule and an Fc fragment. Nature. 1976; 264(5585):415-20. DOI: 10.1038/264415a0. View

Leslie R, MELAMED M, Cohen S . The products from papain and pepsin hydrolyses of guinea-pig immunoglobulins gamma 1G and gamma 2 G. Biochem J. 1971; 121(5):829-37. PMC: 1176671. DOI: 10.1042/bj1210829. View

Hill R, Delaney R, Lebovitz H, Fellows Jr R . Studies on the amino acid sequence of heavy chains from rabbit immunoglobulin G. Proc R Soc Lond B Biol Sci. 1966; 166(1003):159-75. DOI: 10.1098/rspb.1966.0091. View

MacLennan I, CONNELL G, Gotch F . Effector activating determinants on IgG. II. Differentiation of the combining sites for C1q from those for cytotoxic K cells and neutrophils by plasmin digestion of rabbits IgG. Immunology. 1974; 26(2):303-10. PMC: 1423080. View

10.

Colomb M, Porter R . Characterization of a plasmin-digest fragment of rabbit immunoglobulin gamma that binds antigen and complement. Biochem J. 1975; 145(2):177-83. PMC: 1165206. DOI: 10.1042/bj1450177. View

11.

POLJAK R, Amzel L, AVEY H, Chen B, Phizackerley R, Saul F . Three-dimensional structure of the Fab' fragment of a human immunoglobulin at 2,8-A resolution. Proc Natl Acad Sci U S A. 1973; 70(12):3305-10. PMC: 427225. DOI: 10.1073/pnas.70.12.3305. View

12.

Leslie R, Cohen S . Cytophilic activity of IgG2 from sera of unimmunized guinea-pigs. Immunology. 1974; 27(4):577-87. PMC: 1445709. View

13.

Okafor G, Turner M, Hay F . Localisation of monocyte binding site of human immunoglobulin G. Nature. 1974; 248(445):228-30. DOI: 10.1038/248228a0. View

14.

Arend W, Mannik M . The macrophage receptor for IgG: number and affinity of binding sites. J Immunol. 1973; 110(6):1455-63. View

15.

Utsumi S . Stepwise cleavage of rabbit immunoglobulin G by papain and isolation of four types of biologically active Fc fragments. Biochem J. 1969; 112(3):343-55. PMC: 1187714. DOI: 10.1042/bj1120343. View

16.

Yasmeen D, Ellerson J, DORRINGTON K, Painter R . The structure and function of immunoglobulin domains. IV. The distribution of some effector functions among the Cgamma2 and Cgamma3 homology regions of human immunoglobulin G1. J Immunol. 1976; 116(2):518-26. View

17.

OVARY Z, Saluk P, Quijada L, Lamm M . Biologic activities of rabbit immunoglobulin G in relation to domains of the Fc region. J Immunol. 1976; 116(5):1265-71. View

18.

Ramasamy R, Secher D, Adetugbo K . CH3 domain of IgG as binding site to Fc receptor on mouse lymphocytes. Nature. 1975; 253(5493):656. DOI: 10.1038/253656a0. View

19.

Michaelsen T, Wisloff F, Natvig J . Structural requirements in the Fc region of rabbit IgG antibodies necessary to induce cytotoxicity by human lymphocytes. Scand J Immunol. 1975; 4(1):71-8. DOI: 10.1111/j.1365-3083.1975.tb02601.x. View

20.

Guyer R, Koshland M, Knopf P . Immunoglobulin binding by mouse intestinal epithelial cell receptors. J Immunol. 1976; 117(2):587-93. View