Chronic Effects of Botulinum Toxin on Neuromuscular Transmission and Sensitivity to Acetylcholine in Slow and Fast Skeletal Muscle of the Mouse

Overview

Journal J Physiol

Specialty Physiology

Date 1974 Aug 1

PMID 4371301

Citations 23

Authors

D A Tonge

Affiliations

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Abstract

1. A sublethal dose of botulinum toxin (type A) was injected into the muscles of one hind limb of the mouse causing local paralysis.2. Neuromuscular transmission and muscle sensitivity to acetylcholine (ACh) were studied in vitro in soleus and extensor digitorium longus (EDL) from 6 hr to 4 months after the injection of toxin.3. Both soleus and EDL failed to respond to nerve stimulation within 6 hr of the injection of toxin.4. In muscle fibres in which neuromuscular transmission was blocked, subthreshold end-plate potentials (e.p.p.s) were recorded. The amplitude of the e.p.p.s increased during recovery from the effects of the toxin and both muscles contracted in response to nerve stimulation after 2-3 weeks.5. For about 2 months muscles fatigued more rapidly than normal during repetitive nerve stimulation because of the low quantal content of e.p.p.s.6. Supersensitivity to ACh developed in 3-5 days and persisted after the return of neuromuscular transmission. Muscle sensitivity to ACh became normal when the rate of fatigue during nerve stimulation was normal.

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References

THESLEFF S . Supersensitivity of skeletal muscle produced by botulinum toxin. J Physiol. 1960; 151:598-607. PMC: 1363287. DOI: 10.1113/jphysiol.1960.sp006463. View

Del Castillo J, Katz B . The effect of magnesium on the activity of motor nerve endings. J Physiol. 1954; 124(3):553-9. PMC: 1366291. DOI: 10.1113/jphysiol.1954.sp005128. View

Katz B, THESLEFF S . On the factors which determine the amplitude of the miniature end-plate potential. J Physiol. 1957; 137(2):267-78. PMC: 1362978. DOI: 10.1113/jphysiol.1957.sp005811. View

DUCHEN L . An electron microscopic study of the changes induced by botulinum toxin in the motor end-plates of slow and fast skeletal muscle fibres of the mouse. J Neurol Sci. 1971; 14(1):47-60. DOI: 10.1016/0022-510x(71)90129-8. View

Spitzer N . Miniature end-plate potentials at mammalian neuromuscular junctions poisoned by botulinum toxin. Nat New Biol. 1972; 237(70):26-7. DOI: 10.1038/newbio237026a0. View

ELMQVIST D, QUASTEL D . A quantitative study of end-plate potentials in isolated human muscle. J Physiol. 1965; 178(3):505-29. PMC: 1357310. DOI: 10.1113/jphysiol.1965.sp007639. View

Albuquerque E, THESLEFF S . A comparative study of membrane properties of innervated and chronically denervated fast and slow skeletal muscles of the rat. Acta Physiol Scand. 1968; 73(4):471-80. DOI: 10.1111/j.1365-201x.1968.tb10886.x. View

BURGEN A, DICKENS F, ZATMAN L . The action of botulinum toxin on the neuro-muscular junction. J Physiol. 1949; 109(1-2):10-24. PMC: 1392572. DOI: 10.1113/jphysiol.1949.sp004364. View

Miledi R . The acetylcholine sensitivity of frog muscle fibres after complete or partial devervation. J Physiol. 1960; 151:1-23. PMC: 1363214. View

10.

Harris A, Miledi R . The effect of type D botulinum toxin on frog neuromuscular junctions. J Physiol. 1971; 217(2):497-515. PMC: 1331788. DOI: 10.1113/jphysiol.1971.sp009582. View

11.

Martin A . A further study of the statistical composition on the end-plate potential. J Physiol. 1955; 130(1):114-22. PMC: 1363457. DOI: 10.1113/jphysiol.1955.sp005397. View

12.

AMBACHE N . The peripheral action of Cl. botulinum toxin. J Physiol. 1949; 108(2):127-41. PMC: 1392364. View

13.

Del Castillo J, Katz B . Quantal components of the end-plate potential. J Physiol. 1954; 124(3):560-73. PMC: 1366292. DOI: 10.1113/jphysiol.1954.sp005129. View

14.

Lomo T, Rosenthal J . Control of ACh sensitivity by muscle activity in the rat. J Physiol. 1972; 221(2):493-513. PMC: 1331346. DOI: 10.1113/jphysiol.1972.sp009764. View

15.

BROOKS V . An intracellular study of the action of repetitive nerve volleys and of botulinum toxin on miniature end-plate potentials. J Physiol. 1956; 134(2):264-77. PMC: 1359202. DOI: 10.1113/jphysiol.1956.sp005642. View