Regulation of Melanocyte Stimulating Hormone Action at the Receptor Level: Discontinuous Binding of Hormone to Synchronized Mouse Melanoma Cells During the Cell Cycle

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1974 May 1

PMID 4365570

Citations 22

Authors

J M VARGA

A Dipasquale

J Pawelek

J S McGuire

A B LERNER

Affiliations

Soon will be listed here.

Abstract

Melanocyte stimulating hormone coupled to Sepharose effects an increase in tryosinase (EC 1.14.18.1; monophenol monoxygenase) activity of cultivated mouse melanoma cells. Synchronized cells are found to respond to melanocyte stimulating hormone only in the G2 phase of the cell cycle, although their response to cyclic AMP is independent of position in the cell cycle. The binding of (125)I-labeled melanocyte stimulating hormone occurs predominantly in G2. These observations are satisfied by a model in which the hormone can activate adenylate cyclase (EC 4.6.1.1) by binding to a melanocyte stimulating hormone receptor only in G2; the events distal to cyclic AMP production can occur throughout the cell cycle.

Citing Articles

Melanotropic peptide-conjugated beads for microscopic visualization and characterization of melanoma melanotropin receptors.

Sharma S, Jiang J, Hadley M, Bentley D, Hruby V Proc Natl Acad Sci U S A. 1996; 93(24):13715-20.

PMID: 8943000 PMC: 19401. DOI: 10.1073/pnas.93.24.13715.

DNA damage enhances melanogenesis.

Eller M, Ostrom K, Gilchrest B Proc Natl Acad Sci U S A. 1996; 93(3):1087-92.

PMID: 8577719 PMC: 40035. DOI: 10.1073/pnas.93.3.1087.

Combinatorial diffusion assay used to identify topically active melanocyte-stimulating hormone receptor antagonists.

Quillan J, Jayawickreme C, Lerner M Proc Natl Acad Sci U S A. 1995; 92(7):2894-8.

PMID: 7708744 PMC: 42325. DOI: 10.1073/pnas.92.7.2894.

Cell-cycle analysis of insulin binding and internalization on mouse melanoma cells.

Shimizu N, Shimizu Y, Fuller B J Cell Biol. 1981; 88(1):241-4.

PMID: 7009624 PMC: 2111726. DOI: 10.1083/jcb.88.1.241.

Regulation of tyrosinase in human melanocytes grown in culture.

Halaban R, Pomerantz S, Marshall S, Lambert D, LERNER A J Cell Biol. 1983; 97(2):480-8.

PMID: 6411733 PMC: 2112541. DOI: 10.1083/jcb.97.2.480.

References

Abe K, Butcher R, NICHOLSON W, Baird C, Liddle R, LIDDLE G . Adenosine 3',5'-monophosphate (cyclic AMP) as the mediator of the actions of melanocyte stimulating hormone (MSH) and norepinephrine on the frog skin. Endocrinology. 1969; 84(2):362-8. DOI: 10.1210/endo-84-2-362. View

KREIDER J, Rosenthal M, Lengle N . Cyclic adenosine 3',5'-monophosphate in the control of melanoma cell replication and differentiation. J Natl Cancer Inst. 1973; 50(2):555-8. DOI: 10.1093/jnci/50.2.555. View

Pawelek J, Wong G, Sansone M, Morowitz J . Molecular biology of pigment cells. Molecular controls in mammalian pigmentation. Yale J Biol Med. 1973; 46(5):430-43. PMC: 2592014. View

Singer F, Habener J, Greene E, GODIN P, Potts Jr J . Inactivation of calcitonin by specific organs. Nat New Biol. 1972; 237(78):269-70. DOI: 10.1038/newbio237269a0. View

Hunter W, GREENWOOD F . Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962; 194:495-6. DOI: 10.1038/194495a0. View

Atkinson P, Summers D . Purification and properties of HeLa cell plasma membranes. J Biol Chem. 1971; 246(16):5162-75. View

Burger M . A difference in the architecture of the surface membrane of normal and virally transformed cells. Proc Natl Acad Sci U S A. 1969; 62(3):994-1001. PMC: 223697. DOI: 10.1073/pnas.62.3.994. View

Wong G, Pawelek J . Control of phenotypic expression of cultured melanoma cells by melanocyte stimulating hormones. Nat New Biol. 1973; 241(111):213-5. DOI: 10.1038/newbio241213a0. View

Bitensky M, Burstein S . Effects of cyclic adenosine monophosphate and melanocyte-stimulating hormone on frog skin in vitro. Nature. 1965; 208(5017):1282-4. DOI: 10.1038/2081282a0. View

10.

Lande S, LERNER A . The biochemistry of melanotropic agents. Pharmacol Rev. 1967; 19(1):1-20. View

11.

Axen R, Porath J, Ernback S . Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967; 214(5095):1302-4. DOI: 10.1038/2141302a0. View

12.

Martin Jr D, Tomkins G, Granner D . Synthesis and induction of tyrosine aminotransferase in synchronized hepatoma cells in culture. Proc Natl Acad Sci U S A. 1969; 62(1):248-55. PMC: 285980. DOI: 10.1073/pnas.62.1.248. View