Establishment and Maintenance of the Interferon-induced Antiviral State: Studies in Enucleated Cells

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1974 Mar 1

PMID 4362865

Citations 12

Authors

K L Radke

C Colby

J R Kates

H M Krider

D M Prescott

Affiliations

Soon will be listed here.

Abstract

Requirements for the physical presence of the cell's nucleus for the establishment and maintenance of the interferon-induced antiviral state were investigated. Enucleated chicken embryo fibroblasts were obtained by cytochalasin B treatment during centrifugation. The inhibition of vaccinia virus cytoplasmic DNA synthesis, monitored by autoradiography, was used to measure the antiviral activity resulting from interferon treatment. The antiviral state is not established in cells treated with interferon after removal of their nuclei. On the other hand, cells first treated with interferon for 6 or 12 h and then enucleated express the antiviral state. Furthermore, the antiviral state is maintained in enucleated cells for 16 h after enucleation. The antiviral state appears to be more stable in enucleates than in the residual nucleated cells found in the same cultures. Single cells of antiviral populations are found to be either fully permissive or fully restrictive to vaccinia DNA synthesis. The effect of an increasing intracellular multiplicity of infectious virus is to overcome the antiviral cell's block against viral DNA synthesis.

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References

Joklik W, Merigan T . Concerning the mechanism of action of interferon. Proc Natl Acad Sci U S A. 1966; 56(2):558-65. PMC: 224409. DOI: 10.1073/pnas.56.2.558. View

Taylor J . Inhibition of interferon action by actinomycin. Biochem Biophys Res Commun. 1964; 14:447-51. DOI: 10.1016/0006-291x(64)90084-1. View

Jordan G . Quantitative aspects of interferon-induced plaque reduction: kinetics of interferon action. Virology. 1972; 48(2):425-32. DOI: 10.1016/0042-6822(72)90053-0. View

Prescott D, Kates J, Kirkpatrick J . Replication of vaccinia virus DNA in enucleated L-cells. J Mol Biol. 1971; 59(3):505-8. DOI: 10.1016/0022-2836(71)90313-5. View

Joklik W . THE INTRACELLULAR UNCOATING OF POXVIRUS DNA. I. THE FATE OF RADIOACTIVELY-LABELED RABBITPOX VIRUS. J Mol Biol. 1964; 8:263-76. DOI: 10.1016/s0022-2836(64)80136-4. View

Lampson G, TYTELL A, Nemes M, HILLEMAN M . Purification and characterization of chick embryo interferon. Proc Soc Exp Biol Med. 1963; 112:468-78. DOI: 10.3181/00379727-112-28080. View

Ghosh S, Gifford G . Effect of interferon on the dynamics of H3-thymidine incorporation and thymidine kinase induction in chick fibroblast cultures infected with vaccinia virus. Virology. 1965; 27(2):186-92. DOI: 10.1016/0042-6822(65)90158-3. View

Levine S, Magee W, HAMILTON R, MILLER O . Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infections. Virology. 1967; 32(1):33-40. DOI: 10.1016/0042-6822(67)90249-8. View

Fleischmann W, SIMON E . Effect of interferon on virus production from isolated single cells. J Gen Virol. 1973; 20(2):127-37. DOI: 10.1099/0022-1317-20-2-127. View

10.

Friedman R, Sonnabend J, McDevitt H . INTERFERON INHIBITION OF CYTOPLASMIC DNA ACCUMULATION IN VACCINIA VIRUS INFECTION. A RADIOAUTOGRAPHIC STUDY. Proc Soc Exp Biol Med. 1965; 119:551-3. DOI: 10.3181/00379727-119-30235. View

11.

Cairns J . The initiation of vaccinia infection. Virology. 1960; 11:603-23. DOI: 10.1016/0042-6822(60)90103-3. View

12.

Magee W, Levine S, MILLER O, HAMILTON R . Inhibition by interferon of the uncoating of vaccinia virus. Virology. 1968; 35(4):505-11. DOI: 10.1016/0042-6822(68)90280-8. View

13.

Poste G . Enucleation of mammalian cells by cytochalasin B. I. Characterization of anucleate cells. Exp Cell Res. 1972; 73(2):273-86. DOI: 10.1016/0014-4827(72)90049-3. View

14.

Kates J, McAuslan B . Messenger RNA synthesis by a "coated" viral genome. Proc Natl Acad Sci U S A. 1967; 57(2):314-20. PMC: 335507. DOI: 10.1073/pnas.57.2.314. View

15.

Pollack R, Goldman R . Synthesis of infective poliovirus in BSC-1 monkey cells enucleated with cytochalasin B. Science. 1973; 179(4076):915-6. DOI: 10.1126/science.179.4076.915. View

16.

Prescott D, Myerson D, Wallace J . Enucleation of mammalian cells with cytochalasin B. Exp Cell Res. 1972; 71(2):480-5. DOI: 10.1016/0014-4827(72)90322-9. View

17.

HILLER G, Jungwirth C, Bodo G, Schultze B . Biological activity of poly rI:poly rC: effect on poxvirus-specific functions. Virology. 1973; 52(1):22-9. DOI: 10.1016/0042-6822(73)90394-2. View