Nucleoprotein Complexes Containing Replicating Simian Virus 40 DNA: Comparison with Polyoma Nucleoprotein Complexes

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1973 Oct 1

PMID 4359958

Citations 25

Authors

M R Hall

W Meinke

D A Goldstein

Affiliations

Soon will be listed here.

Abstract

Procedures for isolating nucleoprotein complexes containing replicating polyoma DNA from infected mouse cells were used to prepare short-lived nucleoprotein complexes (r-SV40 complexes) containing replicating simian virus 40 (SV40) DNA from infected monkey cells. Like the polyoma complexes, r-SV40 complexes were only partially released from nuclei by cell lysis but could be extracted from nuclei by prolonged treatment with solutions containing Triton X-100. r-SV40 complexes sedimented faster than complexes containing SV40 supercoiled DNA (SV40 complex) in sucrose gradients, and both types of SV40 nucleoprotein complexes sedimented ahead of polyoma complexes containing supercoiled polyoma DNA (py complex). The sedimentation rates of py complex and SV40 complex were 56 and 61S, respectively, based on the sedimentation rate of the mouse large ribosomal subunit as a marker. r-SV40 complexes sedimented as multiple peaks between 56 and 75S. Sedimentation and buoyant density measurements indicated that protein is bound to all forms of SV40 DNA at about the same ratio of protein to DNA (1-2/1) as was reported for polyoma nucleoproteins.

Citing Articles

Many ways to loop DNA.

Griffith J J Biol Chem. 2013; 288(41):29724-35.

PMID: 24005675 PMC: 3795270. DOI: 10.1074/jbc.X113.515981.

Salt-resistant association of simian virus 40 T antigen with simian virus 40 DNA in nucleoprotein complexes.

Rudolph K, Mann K J Virol. 1983; 47(2):276-86.

PMID: 6312066 PMC: 255259. DOI: 10.1128/JVI.47.2.276-286.1983.

Analysis of human cytomegalovirus nucleoprotein complexes.

St Jeor S, Hall C, McGaw C, Hall M J Virol. 1982; 41(1):309-12.

PMID: 6283123 PMC: 256752. DOI: 10.1128/JVI.41.1.309-312.1982.

Properties of nucleoprotein complexes containing replicating polyoma DNA.

Goldstein D, Hall M, Meinke W J Virol. 1973; 12(4):887-900.

PMID: 4359957 PMC: 356708. DOI: 10.1128/JVI.12.4.887-900.1973.

Occurrence of bovine herpesvirus-1 DNA in nucleosomes and chromatin of bovine herpesvirus-1-infected cells: identification of a virion-associated protein in chromatin of infected cells.

Seal B, Martinez J, Hall M, St Jeor S Arch Virol. 1988; 99(3-4):221-36.

PMID: 2835948 DOI: 10.1007/BF01311071.

References

Tashiro Y, Siekevitz P . LOCALIZATION ON HEPATIC RIBOSOMES OF PROTEIN NEWLY SYNTHESIZED IN VIVO. J Mol Biol. 1965; 11:166-73. DOI: 10.1016/s0022-2836(65)80048-1. View

Tashiro Y, Siekevitz P . ULTRACENTRIFUGAL STUDIES ON THE DISSOCIATION OF HEPATIC RIBOSOMES. J Mol Biol. 1965; 11:149-65. DOI: 10.1016/s0022-2836(65)80047-x. View

Hirt B . Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967; 26(2):365-9. DOI: 10.1016/0022-2836(67)90307-5. View

BIELKA H, Welfle H, Bottger M, Forster W . [Structure changes and dissociation of liver ribosomes as a function of the Mg++ concentration]. Eur J Biochem. 1968; 5(2):183-90. DOI: 10.1111/j.1432-1033.1968.tb00356.x. View

Baltimore D, Huang A . Isopycnic separation of subcellular components from poliovirus-infected and normal HeLa cells. Science. 1968; 162(3853):572-4. DOI: 10.1126/science.162.3853.572. View

Hamilton M, RUTH M . The dissociation of rat liver ribosomes by ethylenediaminetetraacetic acid; molecular weights, chemical composition, and buoyant densities of the subunits. Biochemistry. 1969; 8(3):851-6. DOI: 10.1021/bi00831a013. View

Levine A, Kang H, Billheimer F . DNA replication in SV40 infected cells. I. Analysis of replicating SV40 DNA. J Mol Biol. 1970; 50(2):549-68. DOI: 10.1016/0022-2836(70)90211-1. View

Eason R, Vinograd J . Superhelix density heterogeneity of intracellular simian virus 40 deoxyribonucleic acid. J Virol. 1971; 7(1):1-7. PMC: 356070. DOI: 10.1128/JVI.7.1.1-7.1971. View

Green M, MILLER H, Hendler S . Isolation of a polyoma-nucleoprotein complex from infected mouse-cell cultures. Proc Natl Acad Sci U S A. 1971; 68(5):1032-6. PMC: 389107. DOI: 10.1073/pnas.68.5.1032. View

10.

BOURGAUX P, BOURGAUX-RAMOISY D, Seiler P . The replication of the ring-shaped DNA of polyoma virus. II. Identification of molecules at various stages of replication. J Mol Biol. 1971; 59(1):195-206. DOI: 10.1016/0022-2836(71)90421-9. View

11.

Jaenisch R, Mayer A, Levine A . Replicating SV40 molecules containing closed circular template DNA strands. Nat New Biol. 1971; 233(37):72-5. DOI: 10.1038/newbio233072a0. View

12.

White M, Eason R . Nucleoprotein complexes in simian virus 40-infected cells. J Virol. 1971; 8(4):363-71. PMC: 376209. DOI: 10.1128/JVI.8.4.363-371.1971. View

13.

SEBRING E, Kelly Jr T, THOREN M, Salzman N . Structure of replicating simian virus 40 deoxyribonucleic acid molecules. J Virol. 1971; 8(4):478-90. PMC: 376221. DOI: 10.1128/JVI.8.4.478-490.1971. View

14.

Meinke W, Goldstein D . Studies on the structure and formation of polyoma DNA replicative intermediates. J Mol Biol. 1971; 61(3):543-63. DOI: 10.1016/0022-2836(71)90064-7. View

15.

Ozer H . Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells. J Virol. 1972; 9(1):41-51. PMC: 356260. DOI: 10.1128/JVI.9.1.41-51.1972. View

16.

ROBB J, Martin R . Genetic analysis of simian virus 40. 3. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells. J Virol. 1972; 9(6):956-68. PMC: 356401. DOI: 10.1128/JVI.9.6.956-968.1972. View

17.

Green M . Biosynthetic properties of a polyoma nucleoprotein complex: evidence for replication sites. J Virol. 1972; 10(1):32-41. PMC: 356422. DOI: 10.1128/JVI.10.1.32-41.1972. View

18.

BOURGAUX P, BOURGAUX-RAMOISY D . Unwinding of replicating polyoma virus DNA. J Mol Biol. 1972; 70(3):399-413. DOI: 10.1016/0022-2836(72)90548-7. View

19.

Goldstein D, Hall M, Meinke W . Properties of nucleoprotein complexes containing replicating polyoma DNA. J Virol. 1973; 12(4):887-900. PMC: 356708. DOI: 10.1128/JVI.12.4.887-900.1973. View

20.

SCHILDKRAUT C, MARMUR J, Doty P . Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962; 4:430-43. DOI: 10.1016/s0022-2836(62)80100-4. View