Respiration-driven Proton Translocation by Yeast Mitochondria with Differing Efficiencies of Oxidative Phosphorylation

Overview

Journal Biochem J

Specialty Biochemistry

Date 1973 Aug 1

PMID 4357710

Citations 11

Authors

J A Downie

P B Garland

Affiliations

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Abstract

Measurements were made of the stoicheiometry of proton translocation coupled to respiration in mitochondria from Candida utilis where the number of functional energy-conservation sites between intramitochondrial NADH and oxygen was one in a mutant with a novel oxidase (Downie & Garland, 1972), two in sulphate-deficient cells (Haddock & Garland, 1971) or three in glycerol-limited cells (Light & Garland, 1971). The stoicheiometries of protons translocated per atom of oxygen utilized (i.e. -->H(+)/2e(-) ratio; Mitchell, 1966) were close to 2.0, 4.0 and 6.0 respectively. Thus by using the same substrate (intramitochondrial NADH) and oxygen throughout, the -->H(+)/2e(-) ratio is shown to be 2.0 per energy-conservation site when the number of such sites is varied from one to three.

Citing Articles

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The mechanism of proton translocation driven by the respiratory nitrate reductase complex of Escherichia coli.

Jones R, Lamont A, Garland P Biochem J. 1980; 190(1):79-94.

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An antimycin A- and cyanide-resistant variant of Candida utilis arising during copper-limited growth.

Downie J, Garland P Biochem J. 1973; 134(4):1051-61.

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Respiration-driven proton translocation in Escherichia coli.

Lawford H, Haddock B Biochem J. 1973; 136(1):217-20.

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Determination of the efficiency of oxidative phosphorylation in continuous cultures of Aerobacter aerogenes.

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