Differences in the Patterns of Methylation in Rat Liver Ribosomal Ribonucleic Acid After Reaction in Vivo with Methyl Methanesulphonate and NN-dimethylnitrosamine

Overview

Journal Biochem J

Specialty Biochemistry

Date 1972 Sep 1

PMID 4349110

Citations 10

Authors

P J OConnor

M J CAPPS

A W Craig

P D Lawley

S A Shah

Affiliations

Soon will be listed here.

Abstract

1. rRNA was isolated from rat liver at short intervals after the intraperitoneal injection of [(14)C]methyl methanesulphonate (50mg/kg) or NN-di[(14)C]methylnitrosamine (2mg/kg). These doses were chosen to minimize the effects of toxicity. 2. The following methods of hydrolysis of [(14)C]methylated rRNA were employed: enzymic digestion to nucleosides at pH8; alkaline hydrolysis and conversion into nucleosides; acid hydrolysis to bases. 3. The methylation products were analysed by chromatography on columns of Dowex-50 (H(+) form) and Dowex-50 (NH(4) (+) form). 4. With both methylating agents the principal product of methylation was 7-methylguanine. Differences were obtained, however, in the molar proportions of the minor bases 3-methylcytosine, 1-methyladenine and 7-methyladenine. Methylation at the O-6 position of guanine was a significant feature of rRNA obtained from the NN-di[(14)C]methylnitrosamine-treated animals but was not detected in rRNA after treatment with [(14)C]methyl methanesulphonate.

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