Transformation of Rat Liver Cells with Chicken Sarcoma Virus B77 and Murine Sarcoma Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1973 Feb 1

PMID 4347422

Citations 2

Authors

C Altaner

E HLAVAYOVA

Affiliations

Soon will be listed here.

Abstract

Rat liver cells in vitro were transformed with chicken sarcoma virus B77, giving RL(B77) cells, and with murine sarcoma virus (Harvey), giving RL(MSV) cells. Rat liver cells transformed spontaneously in vitro were designated RL cells. In addition, the RL(MSV) cell line was adapted for growth in culture fluid containing 25 mug of 5-bromodeoxyuridine per ml. All cell lines were tumorigenic in 1-wk-old rats. The number of cells needed for induction of tumor growth was 1,000-fold higher in the case of RL(B77) cells in comparison with RL(MSV) cells and RL cells. No production of viral particles from any of the cell lines investigated was detected by plating concentrated supernatant fluid of the cultures on different secondary embryo cells with and without fusion by Sendai virus, by labeling with uridine-5-(3)H, or by assay for deoxyribonucleic acid polymerase activity. The viral genome was rescued by fusion of RL(B77) cells with chicken cells. Chicken sarcoma virus rescued from (RL(B77) cells differed in plating efficiency on duck cells from B77 virus rescued from transformed rat embryo cells. No virus was rescued after fusion of RL(MSV) and RL cells with mouse, rat, or chicken embryo cells. Infectious murine sarcoma virus can be induced by 5-bromodeoxyuridine from RL(MSV) cells.

Citing Articles

Transformation of rat cerebral endothelial cells by Rous sarcoma virus.

Diglio C, Wolfe D, Meyers P J Cell Biol. 1983; 97(1):15-21.

PMID: 6134735 PMC: 2112474. DOI: 10.1083/jcb.97.1.15.

Avian sarcoma virus transformed hamster cells resistant to bromodeoxyuridine and deficient in thymidine kinase activity.

Altaner C, Hladka M, Schlechte H Arch Gesamte Virusforsch. 1973; 41(1):40-51.

PMID: 4352064 DOI: 10.1007/BF01249927.

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