Isolation and Purification of Endotoxin by Hydrolytic Enzymes

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1972 Dec 1

PMID 4344633

Citations 7

Authors

S Lehrer

A Nowotny

Affiliations

Soon will be listed here.

Abstract

Various commercial hydrolases were used in an attempt to degrade the endotoxic lipopolysaccharide macromolecule. Some inert components, such as peptides and nucleic acids, could be removed from endotoxin preparations. As a result, endotoxic activity, measured by pyrogenicity, Shwartzman reaction, and mouse lethality, was increased. The remarkable resistance of endotoxin to hydrolases led to the use of such enzymes for the liberation and purification of endotoxin from whole bacterial cells.

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Release of toxic microvesicles by Actinobacillus actinomycetemcomitans.

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