Immunization of Mice Against Encephalomyocarditis Virus. I. Purification, Concentration, and Inactivation of Encephalomyocarditis Virus

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1972 Oct 1

PMID 4344026

Citations 5

Authors

W J Bogaerts

Affiliations

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Abstract

Methods are described for the concentration, purification, and inactivation of encephalomyocarditis virus grown in L-cell suspension cultures. Virus concentrates were obtained by the precipitation of crude virus lysates with polyethylene glycol and were purified by Genetron extraction. After column chromatography approximately 70% recovery of infectivity and hemagglutination (HA) activity was found. Exposure of the purified virus suspensions to ultraviolet light resulted in reduction of the infectivity by a factor of 10(9) with little or no loss of HA activity. The preparations are being evaluated as vaccines.

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Inactivation of encephalomyocarditis virus in aerosols: fate of virus protein and ribonucleic acid.

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