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2'-O-methylation of Adenosine, Guanosine, Uridine, and Cytidine in RNA of Isolated Rat Liver Nuclei

Overview
Journal Proc Natl Acad Sci U S A
Specialty Science
Date 1972 Jul 1
PMID 4340155
Citations 5
Authors
M B Sporn
Affiliations
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Abstract

Nuclei isolated from rat liver, when incubated with methyl-labeled S-adenosylmethionine, incorporated label into 2'-O-methyladenosine, 2'-O-methylguanosine, 2'-O-methyluridine, and 2'-O-methylcytidine of endogenous nuclear RNA. Addition of ribosomal RNA from wheat germ to the reaction markedly stimulated 2'-O-methylation of total RNA. The relative incorporation of label into the four different 2'-O-methyl ribonucleosides was greatly changed by the addition of wheat germ RNA. There was much more 2'-O-methylation of the purine ribonucleosides, relative to the pyrimidine ribonucleosides, in the reaction stimulated by wheat germ RNA.

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References
1.
Nichols J, LANE B . N-4-methyl-2'-O-methyl cytidine and other methyl-substituted nucleoside constituents of Escherichia coli ribosomal and soluble RNA. Biochim Biophys Acta. 1966; 119(3):649-51. DOI: 10.1016/0005-2787(66)90147-x. View
2.
PERRY R . The nucleolus and the synthesis of ribosomes. Prog Nucleic Acid Res Mol Biol. 1967; 6:219-57. DOI: 10.1016/s0079-6603(08)60528-0. View
3.
Darnell Jr J . Ribonucleic acids from animal cells. Bacteriol Rev. 1968; 32(3):262-90. PMC: 408298. DOI: 10.1128/br.32.3.262-290.1968. View
4.
Sporn M, Lazarus H, Smith J, Henderson W . Studies on nuclear exoribonucleases. 3. Isolation and properties of the enzyme from normal and malignant tissues of the mouse. Biochemistry. 1969; 8(4):1698-706. DOI: 10.1021/bi00832a053. View
5.
Culp L, Brown G . RNA methylases from rat liver nucleoli. Arch Biochem Biophys. 1970; 137(1):222-30. DOI: 10.1016/0003-9861(70)90429-7. View