Activation of the Viral Genome in Simian Virus 40-transformed Nonpermissive Cells by Permissive Cell Extracts

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 May 1

PMID 4338588

Citations 1

Authors

H G Suarez

G E Sonenshein

S Estrade

M F Bourali

R CASSINGENA

P Tournier

Affiliations

Soon will be listed here.

Abstract

Simian virus 40-transformed nonpermissive cells in which neither infectious viral DNA nor virions had previously been detected reproducibly yield low levels of infectious simian virus-40 DNA after treatment with extracts of normal permissive cells. Virions were obtained in one out of 37 experiments. Activation of the viral genome is detected only with normal permissive cell extracts and only in simian virus 40-transformed cell lines that are rescuable by heterokaryon formation or by treatment with chemicals. The activating factor(s) is insensitive to deoxyribonuclease and ribonuclease, but is sensitive to heat at 56 degrees and to proteolytic enzymes.

Citing Articles

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Aubertin A, Hirth C, Travo C, NONNENMACHER H, KIRN A J Virol. 1973; 11(5):694-701.

PMID: 4736109 PMC: 355165. DOI: 10.1128/JVI.11.5.694-701.1973.

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