Manganous Ion As a Spin Label in Studies of Mitochondrial Uptake of Manganese

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1972 Jun 1

PMID 4337705

Citations 15

Authors

T E GUNTER

J S Puskin

Affiliations

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Abstract

Manganous ion (Mn(2+)) has been used as a spin label for studies of divalent cation uptake by rat liver mitochondria. Spin exchange, observed in the electron paramagnetic resonance (EPR) spectrum of a fraction of the transported Mn(2+), shows that this fraction is bound in regions of high local concentration within the mitochondria. The average separation of manganese ions in that fraction is estimated to be 4.0 +/-0.6 A at the time of greatest concentration.

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