Gene Order of the Poliovirus Capsid Proteins

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1972 Mar 1

PMID 4335662

Citations 20

Authors

D Rekosh

Affiliations

Soon will be listed here.

Abstract

Two methods were used to determine the genetic map of the poliovirus capsid proteins. The first method uses pactamycin, a drug which selectively inhibits the initiation of protein synthesis and causes a change in the relative amounts of capsid proteins synthesized. This differential effect on each of the capsid proteins is interpreted as indicating the relative distance of each protein from the initiation site of protein synthesis. The second method involves an analysis of coat precursor molecules released from polyribosomes after a series of short pulses of different length terminated by addition of emetine, a drug which stops all protein synthesis almost immediately after its addition. As the pulse length is increased, each of the capsid proteins within the precursor gains radioactivity with different kinetics. From these kinetics, it is possible to determine the gene order of the capsid proteins within the precursor as well as a rate of protein synthesis. Both methods indicate a gene order for the region of the ribonucleic acid coding for the capsid proteins as (5' --> 3') VP 4 - VP 2 - VP 3 - VP 1.

Citing Articles

Modulation of poliovirus replicative fitness in HeLa cells by deoptimization of synonymous codon usage in the capsid region.

Burns C, Shaw J, Campagnoli R, Jorba J, Vincent A, Quay J J Virol. 2006; 80(7):3259-72.

PMID: 16537593 PMC: 1440415. DOI: 10.1128/JVI.80.7.3259-3272.2006.

In vitro translation of poliovirus RNA: utilization of internal initiation sites in reticulocyte lysate.

Dorner A, Semler B, Jackson R, Hanecak R, Duprey E, Wimmer E J Virol. 1984; 50(2):507-14.

PMID: 6323749 PMC: 255658. DOI: 10.1128/JVI.50.2.507-514.1984.

Protein processing map of poliovirus.

Pallansch M, Kew O, Semler B, Omilianowski D, Anderson C, Wimmer E J Virol. 1984; 49(3):873-80.

PMID: 6321771 PMC: 255549. DOI: 10.1128/JVI.49.3.873-880.1984.

Identification of the initiation site of poliovirus polyprotein synthesis.

Dorner A, Dorner L, Larsen G, Wimmer E, Anderson C J Virol. 1982; 42(3):1017-28.

PMID: 6284987 PMC: 256935. DOI: 10.1128/JVI.42.3.1017-1028.1982.

Cleavage sites within the poliovirus capsid protein precursors.

Larsen G, Anderson C, Dorner A, Semler B, Wimmer E J Virol. 1982; 41(1):340-4.

PMID: 6283126 PMC: 256758. DOI: 10.1128/JVI.41.1.340-344.1982.

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