Evidence for Translation of Viral-specific RNA in Cells of a Mouse Mammary Carcinoma

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Mar 1

PMID 4335063

Citations 16

Authors

R Axel

J Schlom

S Spiegelman

Affiliations

Soon will be listed here.

Abstract

A procedure is described permitting the detection of viral-specific RNA in a mouse mammary tumor. The method involves molecular hybridization with radioactively labeled DNA complementary to the RNA of the mouse mammary-tumor virus. RNA homologous to that of the mammary agent has been found in both the nuclear and polyribosomal fractions of tumor cells. The results imply that the oncogenic information is serving as messenger RNA that directs the synthesis of proteins required for virus production, and perhaps for the maintenance of the neoplastic state. The technology developed is immediately applicable to tumors of human origin.

Citing Articles

Breast-feeding and breast cancer in the offspring.

Ekbom A, Hsieh C, Trichopoulos D, Yen Y, Petridou E, Adami H Br J Cancer. 1993; 67(4):842-5.

PMID: 8471443 PMC: 1968350. DOI: 10.1038/bjc.1993.154.

Incorporation of viral genome in DNA of chronically infected cells.

Zhdanov V, ANDZHAPARIDZE O, BOGOMOLOVA N Experientia. 1974; 30(5):499-501.

PMID: 4833672 DOI: 10.1007/BF01926316.

Nucleic acid hybridization with RNA immobilized on filter paper.

Saxinger W, Ponnamperuma C, Gillespie D Proc Natl Acad Sci U S A. 1972; 69(10):2975-8.

PMID: 4562748 PMC: 389687. DOI: 10.1073/pnas.69.10.2975.

Virus-specific messenger RNA on free and membrane-bound polyribosomes from cells infected with Rauscher leukemia virus.

Gielkens A, Salden M, Bloemendal H Proc Natl Acad Sci U S A. 1974; 71(4):1093-7.

PMID: 4524617 PMC: 388169. DOI: 10.1073/pnas.71.4.1093.

Synthesis of globin ribonucleic acid from duck-reticulocyte chromatin in vitro.

Axel R, Cedar H, Felsenfeld G Proc Natl Acad Sci U S A. 1973; 70(7):2029-32.

PMID: 4516203 PMC: 433658. DOI: 10.1073/pnas.70.7.2029.

References

Penman S . RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966; 17(1):117-30. DOI: 10.1016/s0022-2836(66)80098-0. View

Dinesen J, Haagensen N . [Acute poisoning in a central hospital. 381 consecutive cases from a 4-year period]. Ugeskr Laeger. 1968; 130(18):755-60. View

Lindberg U, Darnell J . SV40-specific RNA in the nucleus and polyribosomes of transformed cells. Proc Natl Acad Sci U S A. 1970; 65(4):1089-96. PMC: 283027. DOI: 10.1073/pnas.65.4.1089. View

Spiegelman S, Burny A, Das M, KEYDAR J, Schlom J, Travnicek M . Characterization of the products of DNA-directed DNA polymerases in oncogenic RNA viruses. Nature. 1970; 227(5258):563-7. DOI: 10.1038/227563a0. View

Hall B, Spiegelman S . Sequence complementarity of T2-DNA and T2-specific RNA. Proc Natl Acad Sci U S A. 1961; 47:137-63. PMC: 221635. DOI: 10.1073/pnas.47.2.137. View