Direct Evidence for a Prostaglandin Receptor and Its Application to Prostaglandin Measurements (rat-adipocytes-antagonists-analogues-mouse Ovary Assay)

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Feb 1

PMID 4333988

Citations 13

Authors

F A KUEHL Jr

J L Humes

Affiliations

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Abstract

A prostaglandin receptor is present in rat adipocytes, as shown by competition studies with [(3)H]-prostaglandin E(1). The affinity of the E prostaglandins for this receptor preparation is greater than that of A and F prostaglandins, an observation consistent with their relative potencies in stimulating cyclic AMP formation in isolated mouse ovaries and various other organs. In the protocol described, binding constants of 3-8 nM were obtained with prostaglandins E(2) and E(1). A sensitivity of 1 pmole makes this method readily applicable for measurement of tissue concentrations of the E prostaglandins.

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References

KUEHL Jr F, Humes J, Mandel L, CIRILLO V, Zanetti M, HAM E . Prostaglandin antagonists: studies on the mode of action of polyphloretin phosphate. Biochem Biophys Res Commun. 1971; 44(6):1464-70. DOI: 10.1016/s0006-291x(71)80250-4. View

Rodbell M . METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS. J Biol Chem. 1964; 239:375-80. View

Sweatman W, COLLIER H . Effects of prostaglandins on human bronchial muscle. Nature. 1968; 217(5123):69. DOI: 10.1038/217069a0. View

Bergstrom S, Carlson L, Weeks J . The prostaglandins: a family of biologically active lipids. Pharmacol Rev. 1968; 20(1):1-48. View

Horton E . Hypotheses on physiological roles of prostaglandins. Physiol Rev. 1969; 49(1):122-61. DOI: 10.1152/physrev.1969.49.1.122. View

Weeks J, Sekhar N, DUCHARME D . Relative activity of prostaglandins E1, A1, E2 and A2 on lipolysis, platelet aggregation, smooth muscle and the cardiovascular system. J Pharm Pharmacol. 1969; 21(2):103-8. DOI: 10.1111/j.2042-7158.1969.tb08205.x. View

Fried J, Santhanakrishnan T, HIMIZU J, Lin C, Ford S, Rubin B . Prostaglandin antagonists: synthesis and smooth muscle activity. Nature. 1969; 223(5202):208-10. DOI: 10.1038/223208a0. View

Beitch B, Eakins K . The effects of prostaglandins on the intraocular pressure of the rabbit. Br J Pharmacol. 1969; 37(1):158-67. PMC: 1703761. DOI: 10.1111/j.1476-5381.1969.tb09533.x. View

SANNER J . Antagonism of prostaglandin E2 by 1-acetyl-2-(8-chloro-10,11-dihydrodibenz (b,f) (1,4) oxazepine-10-carbonyl) hydrazine (SC-19220). Arch Int Pharmacodyn Ther. 1969; 180(1):46-56. View

10.

Maxwell G . The effect of prostaglandin F2alpha upon coronary venous flow and myocardial metabolism. Aust J Exp Biol Med Sci. 1969; 47(6):713-21. DOI: 10.1038/icb.1969.168. View

11.

KUEHL Jr F, Humes J, Tarnoff J, CIRILLO V, HAM E . Prostaglandin receptor site: evidence for an essential role in the action of luteinizing hormone. Science. 1970; 169(3948):883-6. DOI: 10.1126/science.169.3948.883. View

12.

GILMAN A . A protein binding assay for adenosine 3':5'-cyclic monophosphate. Proc Natl Acad Sci U S A. 1970; 67(1):305-12. PMC: 283204. DOI: 10.1073/pnas.67.1.305. View

13.

Thompson C, Los M, Horton E . The separation, identification and estimation of prostaglandins in nanogram quantities by combined gas chromatography-mass spectrometry. Life Sci I. 1970; 9(17):983-8. DOI: 10.1016/0024-3205(70)90121-9. View

14.

Levine L, Van Vunakis H . Antigenic activity of prostaglandins. Biochem Biophys Res Commun. 1970; 41(5):1171-7. DOI: 10.1016/0006-291x(70)90209-3. View

15.

Jaffe B, Smith J, NEWTON W, Parker C . Radioimmunoassay for prostaglandins. Science. 1971; 171(3970):494-6. DOI: 10.1126/science.171.3970.494. View