The Preparation of Brush Borders from the Epithelial Cells of the Guineapig Small Intestine by Zonal Centrifugation

Overview

Journal Histochem J

Specialty Biochemistry

Date 1971 Jan 1

PMID 4329282

Citations 8

Authors

M J Connock

A Elkin

W F POVER

Affiliations

Soon will be listed here.

Citing Articles

Influence of postruminal casein infusion and exogenous glucagon-like peptide 2 administration on the jejunal mucosal transcriptome in cattle.

Trotta R, Swanson K, Klotz J, Harmon D PLoS One. 2024; 19(8):e0308983.

PMID: 39146343 PMC: 11326568. DOI: 10.1371/journal.pone.0308983.

The characterization of rat kidney plasma membranes prepared by zonal centrifugation.

Price R, Taylor D, Robinson D Biochem J. 1972; 129(4):919-28.

PMID: 4347899 PMC: 1174237. DOI: 10.1042/bj1290919.

Zonal rotor analysis of the subcellular localization of alpha-glycerphosphate dehydrogenase, alpha-naphthyl palmitate and beta-naphthyl laurate hydrolases in the mucosa of the guinea-pig small intestine.

Temple N, Martin P, Connock M Histochem J. 1976; 8(2):189-75.

PMID: 1270331 DOI: 10.1007/BF01007166.

Contraction of isolated brush borders from the intestinal epithelium.

Rodewald R, NEWMAN S, Karnovsky M J Cell Biol. 1976; 70(3):541-54.

PMID: 783170 PMC: 2109856. DOI: 10.1083/jcb.70.3.541.

Acid hydrolases in the suckling rat small intestine. II. On the importance of alkaline phosphatase inhibition in the histochemical localization of acid phosphatase activity.

Connock M, Sturdee A Histochem J. 1975; 7(2):103-14.

PMID: 236269 DOI: 10.1007/BF01004555.

References

FORSTNER G, SABESIN S, Isselbacher K . Rat intestinal microvillus membranes. Purification and biochemical characterization. Biochem J. 1968; 106(2):381-90. PMC: 1198514. DOI: 10.1042/bj1060381. View

HUBSCHER G, West G, Brindley D . Studies on the fractionation of mucosal homogenates from the small intestine. Biochem J. 1965; 97(3):629-42. PMC: 1264739. DOI: 10.1042/bj0970629. View

PORTEOUS J . The isolation of purified brush borders from rat small intestine. FEBS Lett. 1968; 1(1):46-49. DOI: 10.1016/0014-5793(68)80015-8. View

Hugon J, Borgers M, Maestracci D . Glucose 6-phosphatase and thiamine pyrophosphatase activities in the jejunal epithelium of the mouse. J Histochem Cytochem. 1970; 18(5):361-4. DOI: 10.1177/18.5.361. View

Miller D, CRANE R . The digestive function of the epithelium of the small intestine. II. Localization of disaccharide hydrolysis in the isolated brush border portion of intestinal epithelial cells. Biochim Biophys Acta. 1961; 52:293-8. DOI: 10.1016/0006-3002(61)90678-3. View

Burton K . A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956; 62(2):315-23. PMC: 1215910. DOI: 10.1042/bj0620315. View

Overton J, Eichholz A, CRANE R . STUDIES ON THE ORGANIZATION OF THE BRUSH BORDER IN INTESTINAL EPITHELIAL CELLS : II. Fine Structure of Fractions of Tris-Disrupted Hamster Brush Borders. J Cell Biol. 2009; 26(3):693-706. PMC: 2106780. DOI: 10.1083/jcb.26.3.693. View

Hugon J, Borgers M . Fine structural localization of acid and alkaline phosphatase activities in the absorbing cells of the duodenum of rodents. Histochemie. 1968; 12(1):42-66. DOI: 10.1007/BF00306346. View

Limbrick A, Finean J . X-ray diffraction and electron-microscope studies of the brush border membrane of guinea pig intestinal epithelial cells. J Cell Sci. 1970; 7(2):373-86. DOI: 10.1242/jcs.7.2.373. View

10.

PENNINGTON R . Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase. Biochem J. 1961; 80:649-54. PMC: 1243280. DOI: 10.1042/bj0800649. View

11.

PORTEOUS J, Clark B . THE ISOLATION AND CHARACTERIZATION OF SUBCELLULAR COMPONENTS OF THE EPITHELIAL CELLS OF RABBIT SMALL INTESTINE. Biochem J. 1965; 96:159-71. PMC: 1206917. DOI: 10.1042/bj0960159. View

12.

Ravin H, Seligman A . Determinant for the specificity of action of pancreatic lipase. Arch Biochem Biophys. 1953; 42(2):337-54. DOI: 10.1016/0003-9861(53)90363-4. View

13.

Wrigglesworth J, POVER W . Subcellular distribution of (lysosomal enzymes) in guinea pig small intestine. Life Sci. 1966; 5(15):1365-71. DOI: 10.1016/0024-3205(66)90111-1. View

14.

HUEBSCHER G, West G . SPECIFIC ASSAYS OF SOME PHOSPHATASES IN SUBCELLULAR FRACTIONS OF SMALL INTESTINAL MUCOSA. Nature. 1965; 205:799-800. DOI: 10.1038/205799a0. View

15.

STETTEN M, BURNETT F . Activation of rat-liver microsomal glucose 6-phosphatase, inorganic pyrophosphatase and inorganic pyrophosphate-glucose phosphotransferase by hydroxyl ion. Biochim Biophys Acta. 1966; 128(2):344-50. DOI: 10.1016/0926-6593(66)90181-0. View

16.

Millington P, Critchley D, Tovell P . A study on the isolation of intestinal brush borders in saline. Z Zellforsch Mikrosk Anat. 1968; 87(3):401-8. DOI: 10.1007/BF00333689. View

17.

HOLT J, Miller D . The localization of phosphomonoesterase and aminopeptidase in brush borders isolated from intestinal epithelial cells. Biochim Biophys Acta. 1962; 58:239-43. DOI: 10.1016/0006-3002(62)91004-1. View

18.

Connock M, Pover W . Catalase particles in the epithelial cells of the guinea-pig small intestine. Histochem J. 1970; 2(5):371-80. DOI: 10.1007/BF01004718. View

19.

Robinson G . The distribution of peptidases in subcellular fractions from the mucosa of the small intestine of the rat. Biochem J. 1963; 88:162-8. PMC: 1203867. DOI: 10.1042/bj0880162. View

20.

Baudhuin P, BEAUFAY H, RAHMAN-LI Y, Sellinger O, WATTIAUX R, Jacques P . Tissue fractionation studies. 17. Intracellular distribution of monoamine oxidase, aspartate aminotransferase, alanine aminotransferase, D-amino acid oxidase and catalase in rat-liver tissue. Biochem J. 1964; 92(1):179-84. PMC: 1215456. DOI: 10.1042/bj0920179. View