Phosphorylation of Ribosome-associated Protein by an Adenosine 3':5'-cyclic Monophosphate-dependent Protein Kinase: Location of the Microsomal Receptor and Protein Kinase

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1971 May 1

PMID 4325002

Citations 17

Authors

G M Walton

G N Gill

I B Abrass

L D GARREN

Affiliations

Soon will be listed here.

Abstract

In the adrenal cortex, the adenosine 3':5'-cyclic monophosphate (cAMP)-receptor protein and the cAMP-dependent protein kinase are located in both the cytosol and endoplasmic reticulum. The cAMP-dependent protein kinase from the cytosol catalyzes the phosphorylation of ribosome-associated protein. Ribosomes washed in 0.5 M NH(4)Cl retain the substrate of the protein kinase reaction, but are dependent on the NH(4)Cl extract for in vitro protein synthesis. Dissociation of the 80S ribosomes by 0.88 M KCl, however, releases the ribosome-associated (protein) substrate of the phosphorylation reaction.

Citing Articles

Physiological control of phosphorylation ribosomal protein S6 in Mucor racemosus.

Larsen A, Sypherd P J Bacteriol. 1980; 141(1):20-5.

PMID: 7353998 PMC: 293523. DOI: 10.1128/jb.141.1.20-25.1980.

GTP-sensitive phosphorylation of proteins in a postmitochondrial supernatant from rat brainstem affected by ACTH1-24.

van Dijk A, King G, Schotman P, Gispen W Neurochem Res. 1981; 6(8):847-61.

PMID: 6273759 DOI: 10.1007/BF00965043.

Influence of ACTH on phosphorylation of ribosomal proteins from rat adrenal glands.

YUDAEV N, KREKHOVA M, Chekhranova M Neurosci Behav Physiol. 1980; 10(1):85-92.

PMID: 6247670 DOI: 10.1007/BF01182242.

Alteration of ribosomal protein S4 by mutation linked to kasugamycin-resistance in Escherichia coli.

Zimmermann R, Ikeya Y, Sparling P Proc Natl Acad Sci U S A. 1973; 70(1):71-5.

PMID: 4567337 PMC: 433186. DOI: 10.1073/pnas.70.1.71.

Chemical and immunological studies of liver microsomes from mouse mutants with ultrastructurally abnormal hepatic endoplasmic reticulum.

Erickson R, Siekevitz P, Jacobs K, Gluecksohn-Waelsch S Biochem Genet. 1974; 12(1):81-95.

PMID: 4372989 DOI: 10.1007/BF00487530.

References

GASIOR E, MOLDAVE K . RESOLUTION OF AMINOACYL-TRANSFERRING ENZYMES FROM RAT LIVER BY MOLECULAR SIEVE CHROMATOGRAPHY. J Biol Chem. 1965; 240:3346-52. View

Martelo O, Woo S, Reimann E, Davie E . Effect of protein kinase on ribonucleic acid polymerase. Biochemistry. 1970; 9(24):4807-13. DOI: 10.1021/bi00826a027. View

Walton G, GARREN L . An assay for adenosine 3',5'-cyclic monophosphate based on the association of the nucleotide with a partially purified binding protein. Biochemistry. 1970; 9(21):4223-9. DOI: 10.1021/bi00823a026. View

FERGUSON Jr J, Morita Y . RNA SYNTHESIS AND ADRENOCORTICOTROPIN RESPONSIVENESS. Biochim Biophys Acta. 1964; 87:348-50. DOI: 10.1016/0926-6550(64)90234-8. View

Gill G, GARREN L . On the mechanism of action of adrenocorticotropic hormone: the binding of cyclic-3',5'-adenosine monophosphate to an adrenal cortical protein. Proc Natl Acad Sci U S A. 1969; 63(2):512-9. PMC: 223593. DOI: 10.1073/pnas.63.2.512. View

Kerwar S, Spears C, Weissbach H . Studies on the initiation of protein synthesis in animal tissues. Biochem Biophys Res Commun. 1970; 41(1):78-84. DOI: 10.1016/0006-291x(70)90471-7. View

Gill G, GARREN L . A cyclic-3',5'-adenosine monophosphate dependent protein kinase from the adrenal cortex: comparison with a cyclic AMP binding protein. Biochem Biophys Res Commun. 1970; 39(3):335-43. DOI: 10.1016/0006-291x(70)90581-4. View

Martin T, Wool I . Formation of active hybrids from subunits of muscle ribosomes from normal and diabetic rats. Proc Natl Acad Sci U S A. 1968; 60(2):569-74. PMC: 225085. DOI: 10.1073/pnas.60.2.569. View

Prichard P, Gilbert J, Shafritz D, Anderson W . Factors for the initiation of haemoglobin synthesis by rabbit reticulocyte ribosomes. Nature. 1970; 226(5245):511-4. DOI: 10.1038/226511a0. View

10.

Corbin J, Reimann E, Walsh D, KREBS E . Activation of adipose tissue lipase by skeletal muscle cyclic adenosine 3',5'- monophosphate-stimulated protein kinase. J Biol Chem. 1970; 245(18):4849-51. View

11.

Bandurski R, AXELROD B . The chromatographic identification of some biologically important phosphate esters. J Biol Chem. 1951; 193(1):405-10. View

12.

Glynn I, Chappell J . A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity. Biochem J. 1964; 90(1):147-9. PMC: 1202534. DOI: 10.1042/bj0900147. View

13.

HAYNES Jr R, BERTHET L . Studies on the mechanism of action of the adrenocorticotropic hormone. J Biol Chem. 1957; 225(1):115-24. View

14.

Weller M, Rodnight R . Stimulation by cyclic AMP of intrinsic protein kinase activity in ox brain membrane preparations. Nature. 1970; 225(5228):187-8. DOI: 10.1038/225187a0. View

15.

Soderling T, Hickenbottom J, Reimann E, Hunkeler F, Walsh D, KREBS E . Inactivation of glycogen synthetase and activation of phosphorylase kinase by muscle adenosine 3',5'-monophosphate-dependent protein kinases. J Biol Chem. 1970; 245(23):6317-28. View

16.

HAYNES Jr R, KORITZ S, PERON F . Influence of adenosine 3',5'-monophosphate on corticoid production by rat adrenal glands. J Biol Chem. 1959; 234(6):1421-3. View

17.

LANGAN T . Histone phosphorylation: stimulation by adenosine 3',5'-monophosphate. Science. 1968; 162(3853):579-80. DOI: 10.1126/science.162.3853.579. View

18.

Fleck A, MUNRO H . The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation. Biochim Biophys Acta. 1962; 55:571-83. DOI: 10.1016/0006-3002(62)90836-3. View

19.

Grahame-Smith D, Butcher R, NEY R, SUTHERLAND E . Adenosine 3',5'-monophosphate as the intracellular mediator of the action of adrenocorticotropic hormone on the adrenal cortex. J Biol Chem. 1967; 242(23):5535-41. View

20.

DeLange R, KEMP R, Riley W, Cooper R, KREBS E . Activation of skeletal muscle phosphorylase kinase by adenosine triphosphate and adenosine 3',5'-monophosphate. J Biol Chem. 1968; 243(9):2200-8. View