Analysis of Clostridium Botulinum Toxigenic Types A, B, and E for Fatty and Carbohydrate Content

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Mar 1

PMID 4324194

Citations 2

Authors

K J Fugate

L B Hansen

O White

Affiliations

Soon will be listed here.

Abstract

Lyophilized, 48-hr log-phase vegetative cells were extracted with chloroform-methanol (2:1, v/v) and ethanol-ether (3:1, v/v) and then saponified with methanolic KOH. Gas-liquid chromatography of the methyl esters of extractable fatty acids revealed distinctive "pattern profiles" of Clostridium botulinum toxigenic types "A," "B," and "E." C. perfringens type "A" and Escherichia coli strain "B" were also studied in a similar manner and were found to give pattern profiles which were distinct even from those obtained for the C. botulinum microorganisms. Amino sugar content of the five microorganisms was determined by using a Beckman amino acid analyzer. The molar ratio of glucosamine to that of galactosamine was found to be of further assistance in distinguishing the individual microorganisms.

Citing Articles

Applications of cellular fatty acid analysis.

Welch D Clin Microbiol Rev. 1991; 4(4):422-38.

PMID: 1747860 PMC: 358210. DOI: 10.1128/CMR.4.4.422.

Stable gas-liquid chromatography column for fatty acid analysis.

Hansen L, Kuhnley L, Kenner C Appl Environ Microbiol. 1977; 33(2):480-1.

PMID: 322613 PMC: 170706. DOI: 10.1128/aem.33.2.480-481.1977.

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