Induction and Repression of L-arabinose Isomerase in Salmonella Typhimurium

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1971 Apr 1

PMID 4323960

Citations 5

Authors

A K Bhattacharya

M Chakravorty

Affiliations

Soon will be listed here.

Abstract

As with other inducible enzymes, the induced synthesis of l-arabinose isomerase (l-arabinose ketol isomerase, EC 5.3.1.4) in Salmonella typhimurium is subject to catabolite repression. Of the three catabolite repressors tested, glucose produces maximum repression. Analogues of catabolite repressors like 2-deoxy-d-glucose and d-fucose also inhibit the synthesis of the enzyme. The catabolite repression is completely reversed in the presence of 1.5 x 10(-3)m cyclic 3',5'-adenosine monophosphate (AMP). The maximum repression is produced in glucose-grown cells in glucose-containing induction medium. Cyclic 3',5-AMP reverses this repression provided that the cells are treated with ethylenediaminetetraacetic acid (EDTA). In normal cells, cyclic 3',5'-AMP has no effect on the induction but in EDTA-treated cells the cyclic nucleotide enhances synthesis of the enzyme. The inhibition produced by d-fucose cannot be reversed by cyclic 3',5'-AMP. d-Fucose competes with the inducer l-arabinose in some step(s) involved in the process of induction.

Citing Articles

Isolation of ara-lac gene fusions in Salmonella typhimurium LT2 by using transducing bacteriophage Mu d (Apr lac).

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Linkage map of Salmonella typhimurium, edition IV.

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Induction of the ara operon of Escherichia coli B-r.

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Evidence against the presence of 3',5'-cyclic adenosine monophosphate and relevant enzymes in Lactobacillus plantarum.

Sahyoun N, Durr I J Bacteriol. 1972; 112(1):421-6.

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