Article: A preliminary report on the thymidine labeling indices and kinetics of cell proliferation in selected Morris hepatomas

The 1 hour thymidine labeling indices have been determined for 8 hepatomas which have growth rates which vary by a factor of 14. The indices for these tumors vary only by a factor of 4. Little correlation was found. Preliminary results have been obtained on the kinetics of cell proliferation of the rapidly growing Hepatoma H-35tc(2). The tumor transfer time is 0.7 months and the growth rate is 7.0 cm. per month. The calculated values for times in different phases of the cell cycle for H-35tc(2), assuming a log normal distribution for phase duration, were as follows: T(g1) (Gap I)-11.0 hours; T(s) (DNA synthetic period)-6.6 hours; T(g2) (Gap II)-4.2 hours; T(m) (mitotic time)-0.4 hours. Therefore, the total time, T(c), for one cell cycle was calculated to be 22.2 hours. The potential doubling time was calculated to be 43 hours. The GF (growth fraction) was estimated to be 53 per cent which would suggest that approximately one-half the total cell population is nonproliferating.

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References

1.

LOONEY W, CHANG L, BANGHART F . The rate of DNA replication at the molecular, chromosomal, cellular, and intercellular levels in regenerating rat liver. Proc Natl Acad Sci U S A. 1967; 57(4):972-8. PMC: 224642. DOI: 10.1073/pnas.57.4.972. View

2.

CHANG L, MORRIS H, LOONEY W . Rates of incorporation of tritium-labeled thymidine into the mitochondrial and nuclear DNA of normal rat liver, regenerating liver, and four hepatomas with different growth rates and their host livers. Br J Cancer. 1968; 22(4):860-6. PMC: 2008370. DOI: 10.1038/bjc.1968.101. View

3.

Hofer K, Rosenoff S, Prensky W, HUGHES W . Spontaneous and amethopterin-induced death of L1210 leukaemia cells in vivo. Nature. 1969; 221(5180):576-7. DOI: 10.1038/221576a0. View

4.

LESHER S . Compensatory reactions in intestinal crypt cells after 300 Roentgens of cobalt-60 gamma irradiation. Radiat Res. 1967; 32(3):510-9. View

5.

Dethlefsen L, Prewitt J, Mendelsohn M . Analysis of tumor growth curves. J Natl Cancer Inst. 1968; 40(2):389-405. DOI: 10.1093/jnci/40.2.389. View

6.

Nowell P, MORRIS H, POTTER V . Chromosomes of "minimal deviation" hepatomas and some other transplantable rat tumors. Cancer Res. 1967; 27(9):1565-79. View

7.

MORRIS H . Studies on the development, biochemistry, and biology of experimental hepatomas. Adv Cancer Res. 1965; 9:227-302. DOI: 10.1016/s0065-230x(08)60448-0. View

8.

Denekamp J . The cellular proliferation kinetics of animal tumors. Cancer Res. 1970; 30(2):393-400. View

9.

Mendelsohn M . Autoradiographic analysis of cell proliferation in spontaneous breast cancer of C3H mouse. III. The growth fraction. J Natl Cancer Inst. 1962; 28:1015-29. View

10.

STEEL G, BENSTED J . In vitro studies of cell proliferation in tumours. I. Critical appraisal of methods and theoretical considerations. Eur J Cancer (1965). 1965; 1(3):275-9. DOI: 10.1016/0014-2964(65)90060-5. View

11.

Post J, Hoffman J . THE REPLICATION TIME AND PATTERN OF CARCINOGEN--INDUCED HEPATOMA CELLS. J Cell Biol. 1964; 22:341-50. PMC: 2106459. DOI: 10.1083/jcb.22.2.341. View

12.

Bresciani F . Cell proliferation in cancer. Eur J Cancer (1965). 1968; 4(4):343-66. DOI: 10.1016/0014-2964(68)90025-x. View

13.

STEEL G, Adams K, Barrett J . Analysis of the cell population kinetics of transplanted tumours of widely-differing growth rate. Br J Cancer. 1966; 20(4):784-800. PMC: 2008152. DOI: 10.1038/bjc.1966.90. View

A Preliminary Report on the Thymidine Labeling Indices and Kinetics of Cell Proliferation in Selected Morris Hepatomas