Viremia and Virus Measurements of Rabbit Pox in CV-1 Cells

Overview

Journal Appl Microbiol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1970 May 1

PMID 4316272

Citations 2

Authors

C T Cho

T Locke

H A Wenner

Affiliations

Soon will be listed here.

Abstract

Rabbit pox virus (RPV) produced cytopathic effect (CPE) in five types of cells grown in tissue cultures. The CPE on CV-1 cells was characterized by cell fusion and lysis. The CV-1 line is a useful and sensitive cell culture for measuring concentrations of RPV in blood and tissues of infected rabbits. Viremia was detected between the 2nd and 4th days after parenteral infection. By the 6th and 7th days, the concentration of RPV in various tissues ranged from 10(5-3) to 10(8-5) TCID(50)/g. Cross-reactivity was demonstrated by the fluorescent-antibody technique between rabbit pox, vaccinia, and monkey pox viruses.

Citing Articles

Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrick™ design, serum-free virus production and microcarrier-based cultivation of CV-1 cells.

Liu S, Ruban L, Wang Y, Zhou Y, Nesbeth D Heliyon. 2017; 3(2):e00238.

PMID: 28203643 PMC: 5294666. DOI: 10.1016/j.heliyon.2017.e00238.

Monkeypox virus.

Cho C, Wenner H Bacteriol Rev. 1973; 37(1):1-18.

PMID: 4349404 PMC: 413801. DOI: 10.1128/br.37.1.1-18.1973.

References

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Wenner H, Cho C, Bolano C, KAMITSUKA P . Studies on the pathogenesis of monkey pox. II. Dose-response and virus dispersion. Arch Gesamte Virusforsch. 1969; 27(2):166-78. DOI: 10.1007/BF01249641. View

Wenner H, Bolano C, Cho C, KAMITSUKA P . Studies on the pathogenesis of monkey pox. 3. Histopathological lesions and sites of immunofluorescence. Arch Gesamte Virusforsch. 1969; 27(2):179-97. DOI: 10.1007/BF01249642. View

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