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The Effect of PH on the Characteristics of the Binding of Nicotinamide-adenine Dinucleotide by Nicotinamide-adenine Dinucleotide-specific Isocitrate Dehydrogenase from Pea Mitochondria

Overview
Journal Biochem J
Specialty Biochemistry
Date 1970 Mar 1
PMID 4315048
Citations 2
Authors
G F Cox
D D Davies
Affiliations
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Abstract

1. The effect of pH on the V(max.) and concentration of NAD(+) at half-maximum velocity at a constant isocitrate concentration was examined, and the results were related to the requirements for binding of H(+) ions to the enzyme. 2. The effect of varying the NAD(+) concentration on the pH optimum with constant isocitrate concentration was studied. 3. A comparison has been made between the effect of isocitrate concentration on the characteristics of binding of NAD(+) and the effect of NAD(+) concentration on the characteristics of isocitrate binding at three different pH values. 4. The mechanistic and metabolic significance of these studies is considered.

Citing Articles

NADP-Utilizing Enzymes in the Matrix of Plant Mitochondria.

Rasmusson A, Moller I Plant Physiol. 1990; 94(3):1012-8.

PMID: 16667790 PMC: 1077335. DOI: 10.1104/pp.94.3.1012.

Nicotinamide adenine dinucleotide phosphate-specific isocitrate dehydrogenase from a higher plant. Isolation and charactertization.

Omran R, Dennis D Plant Physiol. 1971; 47(1):43-7.

PMID: 5543783 PMC: 365808. DOI: 10.1104/pp.47.1.43.

References
1.
Atkinson D, HATHAWAY J, Smith E . KINETICS OF REGULATORY ENZYMES. KINETIC ORDER OF THE YEAST DIPHOSPHOPYRIDINE NUCLEOTIDE ISOCITRATE DEHYDROGENASE REACTION AND A MODEL FOR THE REACTION. J Biol Chem. 1965; 240:2682-90. View
2.
SANWAL B, STACHOW C . ALLOSTERIC ACTIVATION OF NICOTINAMIDE-ADENINE DINUCLEOTIDE SPECIFIC ISOCITRATE DEHYDROGENASE OF NEUROSPORA. Biochim Biophys Acta. 1965; 96:28-44. DOI: 10.1016/0005-2787(65)90606-4. View
3.
Cox G, Davies D . Nicotinamide-adenine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria. Purification and properties. Biochem J. 1967; 105(2):729-34. PMC: 1198371. DOI: 10.1042/bj1050729. View
4.
Cox G, Davies D . The effects of pH and citrate on the activity of nicotinamideA-denine dinucleotide-specific isocitrate dehydrogenase from pea mitochondria. Biochem J. 1969; 113(5):813-20. PMC: 1184771. DOI: 10.1042/bj1130813. View
5.
Coultate T, Dennis D . Regulatory properties of a plant NAD: isocitrate dehydrogenase. The effect of inorganic ions. Eur J Biochem. 1969; 7(2):153-8. DOI: 10.1111/j.1432-1033.1969.tb19586.x. View