Alpha 2.0
Login Register
» Articles » PMID: 4311459

The Preferential Release of Newly Synthesized Transmitter by a Sympathetic Ganglion

Overview
Journal J Physiol
Specialty Physiology
Date 1969 Nov 1
PMID 4311459
Citations 28
Authors
B Collier
Affiliations
Soon will be listed here.
Abstract

1. The acetylcholine (ACh) store of cat's superior cervical ganglia was replaced with radioactive ACh by perfusion, during stimulation, with [(3)H]choline-Locke solution. Perfusion was continued with Locke containing unlabelled choline (Ch) (in physiological concentration) and the release of labelled and unlabelled ACh was measured.2. Electrical stimulation of the preganglionic sympathetic nerve (20/sec or 5/sec), or stimulation by perfusing with raised K, released ACh that had a lower specific radioactivity than ganglionic ACh. The proportion of released ACh that was labelled was slightly higher when stimulation was at lower frequency or by K.3. Preganglionic nerve stimulation released, in the first few minutes, ACh that had a specific activity 70-80% of ganglionic ACh, but after 5 min the proportion of label in the released ACh fell to 35-45% of that in the ganglion.4. It is concluded that newly synthesized ACh is released before equilibration with preformed stores, and the significance of this to the mechanism of transmitter release is discussed.

Citing Articles

Analysis of Caenorhabditis elegans acetylcholine synthesis mutants reveals a temperature-sensitive requirement for cholinergic neuromuscular function.

Duerr J, McManus J, Crowell J, Rand J Genetics. 2021; 218(4).

PMID: 34028515 PMC: 9335933. DOI: 10.1093/genetics/iyab078.

A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags.

Muller A, Neukam M, Ivanova A, Sonmez A, Munster C, Kretschmar S Sci Rep. 2017; 7(1):23.

PMID: 28154417 PMC: 5428382. DOI: 10.1038/s41598-017-00033-x.

Activity-dependent vesicular monoamine transporter-mediated depletion of the nucleus supports somatic release by serotonin neurons.

Colgan L, Putzier I, Levitan E J Neurosci. 2009; 29(50):15878-87.

PMID: 20016104 PMC: 2796554. DOI: 10.1523/JNEUROSCI.4210-09.2009.

Mechanism of the noradrenergic motor control on the lower oesophageal sphincter in the cat.

Gonella J, Niel J, Roman C J Physiol. 1980; 306:251-60.

PMID: 7463362 PMC: 1283004. DOI: 10.1113/jphysiol.1980.sp013395.

The morphology of synaptic profiles in explants of foetal and neonatal mouse cerebral cortex maintained in a magnesium-enriched environment.

Bird M Cell Tissue Res. 1980; 206(1):115-22.

PMID: 7357589 DOI: 10.1007/BF00233612.

References
1.
BLAKELEY A, Brown G, Dearnaley D, Harrison V . The effect of nerve stimulation on the synthesis of 3H-noradrenaline from 3H-tyrosine in the isolated blood-perfused cat spleen. J Physiol. 1969; 200(1):59P-60P. View
2.
EMMELIN N, MacINTOSH F . The release of acetylcholine from perfused sympathetic ganglia and skeletal muscles. J Physiol. 1956; 131(2):477-96. PMC: 1363444. DOI: 10.1113/jphysiol.1956.sp005477. View
3.
PERRY W . Acetylcholine release in the cat's superior cervical ganglion. J Physiol. 1953; 119(4):439-54. PMC: 1392724. DOI: 10.1113/jphysiol.1953.sp004858. View
4.
LILEY A . The effects of presynaptic polarization on the spontaneous activity at the mammalian neuromuscular junction. J Physiol. 1956; 134(2):427-43. PMC: 1359215. DOI: 10.1113/jphysiol.1956.sp005655. View
5.
BIRKS R . THE ROLE OF SODIUM IONS IN THE METABOLISM OF ACETYLCHOLINE. Can J Biochem Physiol. 1963; 41:2573-97. View