The Diphosphoinositide Kinase of Rat Brain

Overview

Journal Biochem J

Specialty Biochemistry

Date 1968 Feb 1

PMID 4295336

Citations 19

Authors

M Kai

J G Salway

J N HAWTHORNE

Affiliations

Soon will be listed here.

Abstract

1. The supernatant fraction of adult rat brain contains a diphosphoinositide kinase. 2. Formation of triphosphoinositide by the enzyme in the presence of ATP and Mg(2+) ions was shown with labelled ATP or labelled diphosphoinositide. 3. The kinase was also activated by Ca(2+), Mn(2+) and Co(2+) ions, but to a smaller extent than by Mg(2+) ions. 4. In the presence of optimum Mg(2+) ion concentration the enzyme was inhibited by Ca(2+) ions. 5. Activity did not depend on thiol groups and the pH optimum was 7.3. 6. The dialysed supernatant fraction had no diglyceride kinase activity and negligible phosphatidylinositol kinase activity. 7. Triphosphoinositide phosphomonoesterase was present but showed little activity under the conditions used to assay the kinase. 8. Diphosphoinositide kinase was purified by ammonium sulphate fractionation, ethanol treatment and chromatography on Sephadex G-200. 9. This purification removed much of the triphosphoinositide phosphomonoesterase.

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References

Hendrickson H, Ballou C . ION EXCHANGE CHROMATOGRAPHY OF INTACT BRAIN PHOSPHOINOSITIDES ON DIETHYLAMINOETHYL CELLULOSE BY GRADIENT SALT ELUTION IN A MIXED SOLVENT SYSTEM. J Biol Chem. 1964; 239:1369-73. View

EICHBERG J, Dawson R . Polyphosphoinositides in myelin. Biochem J. 1965; 96(3):644-50. PMC: 1207198. DOI: 10.1042/bj0960644. View

Salway J, Kai M, HAWTHORNE J . Triphosphoinositide phosphomonoesterase activity in nerve cellbodies, neuroglia and subcellular fractions from whole rat brain. J Neurochem. 1967; 14(10):1013-24. DOI: 10.1111/j.1471-4159.1967.tb09512.x. View

COLODZIN M, Kennedy E . Biosynthesis of diphosphoinositide in brain. J Biol Chem. 1965; 240(10):3771-80. View

King E . The colorimetric determination of phosphorus. Biochem J. 1932; 26(2):292-7. PMC: 1260904. DOI: 10.1042/bj0260292. View

Rose S . Preparation of enriched fractions from cerebral cortex containing isolated, metabolically active neuronal and glial cells. Biochem J. 1967; 102(1):33-43. PMC: 1270206. DOI: 10.1042/bj1020033. View

HOKIN M, HOKIN L . The synthesis of phosphatidic acid from diglyceride and adenosine triphosphate in extracts of brain microsomes. J Biol Chem. 1959; 234(6):1381-6. View

Prottey C, HAWTHORNE J . The lipids of mammalian pancreas. Biochem J. 1966; 101(1):191-6. PMC: 1270082. DOI: 10.1042/bj1010191. View

GLOCK G, McLean P . Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem J. 1953; 55(3):400-8. PMC: 1269290. DOI: 10.1042/bj0550400. View

10.

Michell R, HAWTHORNE J . The site of diphosphoinositide synthesis in rat liver. Biochem Biophys Res Commun. 1965; 21(4):333-8. DOI: 10.1016/0006-291x(65)90198-1. View

11.

Nyman M, Whittaker V . The distribution of adenosine triphosphate in subcellular fractions of brain tissue. Biochem J. 1963; 87:248-55. PMC: 1201883. DOI: 10.1042/bj0870248. View

12.

Dawson R, Thompson W . The triphosphoinositide phosphomonoesterase of brain tissue. Biochem J. 1964; 91(2):244-50. PMC: 1202879. DOI: 10.1042/bj0910244. View

13.

Dawson R . 'Phosphatido-peptide'-like complexes formed by the interaction of calcium triphosphoinositide with protein. Biochem J. 1965; 97(1):134-8. PMC: 1264552. DOI: 10.1042/bj0970134. View

14.

Goodman D . Preparation of human serum albumin free of long-chain fatty acids. Science. 1957; 125(3261):1296-7. DOI: 10.1126/science.125.3261.1296. View

15.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

16.

Durell J, SODD M . Studies on the acetylcholine-stimulated incorporation of radioactive inorganic orthophosphate into the phospholipid of brain particulate preparations. II. Subcellular distribution of enzymic activity. J Neurochem. 1966; 13(6):487-91. DOI: 10.1111/j.1471-4159.1966.tb09863.x. View

17.

SHELTAWY A, Dawson R . The polyphosphoinositides and other lipids of peripheral nerves. Biochem J. 1966; 100(1):12-8. PMC: 1265086. DOI: 10.1042/bj1000012. View

18.

Kai M, White G, HAWTHORNE J . The phosphatidylinositol kinase of rat brain. Biochem J. 1966; 101(2):328-37. PMC: 1270113. DOI: 10.1042/bj1010328. View