Influence of Temperature Acclimatization on the Temperature-dependence and Ouabain-sensitizing of Goldfish Intestinal Adenosine Triphosphatase

Overview

Journal Biochem J

Specialty Biochemistry

Date 1967 Oct 1

PMID 4293960

Citations 11

Authors

M W Smith

Affiliations

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Abstract

1. Homogenates of goldfish intestinal mucosa were separated into various fractions by differential centrifugation. Both adenosine-triphosphatase and beta-glycerophosphatase activities were found to be concentrated mainly in a membrane fraction which sedimented after 1200000g-min. 2. This membrane adenosine-triphosphatase system was activated by Na(+)+K(+) and inhibited by ouabain. 3. The ouabain-sensitive adenosine-triphosphatase activity was high and the ouabain-insensitive activity low in membrane fractions prepared from fish acclimatized previously to 8 degrees . The opposite was true for fish acclimatized to 30 degrees . 4. The Arrhenius plots of ouabain-sensitive and ouabain-insensitive adenosine-triphosphatase activities, measured from 5 degrees to 30 degrees , showed discontinuities at incubation temperatures that varied with the previous acclimatization temperature of the fish. 5. It is considered that modification of the membrane adenosine-triphosphatase system in goldfish intestinal mucosa may serve to regulate Na(+) transport at different environmental temperatures.

Citing Articles

Influence of temperature acclimatization on the ionic activation of goldfish intestinal adenosine triphosphatase.

Smith M, Colombo V, Munn E Biochem J. 1968; 107(5):691-8.

PMID: 16742591 PMC: 1198722. DOI: 10.1042/bj1070691.

The effect of temperature on catalytic and regulatory functions of pyruvate kinases of the rainbow trout and the antarctic fish Trematomus bernacchii.

Somero G, Hochachka P Biochem J. 1968; 110(3):395-400.

PMID: 5701664 PMC: 1187363. DOI: 10.1042/bj1100395.

An investigation of Na efflux from the toad oocyte using cold shock as a technic.

BITTAR E Experientia. 1971; 27(3):268-70.

PMID: 5546636 DOI: 10.1007/BF02138138.

Effect of temperature acclimatization on the fatty acid composition of goldfish intestinal lipids.

Kemp P, Smith M Biochem J. 1970; 117(1):9-15.

PMID: 4316092 PMC: 1178825. DOI: 10.1042/bj1170009.

The temperature dependence of activation by phosphatidylserine of the sodium pump adenosine triphosphatase.

Priestland R, Whittam R J Physiol. 1972; 220(2):353-61.

PMID: 4259203 PMC: 1331707. DOI: 10.1113/jphysiol.1972.sp009711.

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