The Biosynthesis in Vitro of Keratan Sulphate in Bovine Cornea

Overview

Journal Biochem J

Specialty Biochemistry

Date 1972 Jun 1

PMID 4263792

Citations 8

Authors

C J Handley

C F Phelps

Affiliations

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Abstract

1. Bovine corneas were incubated in vitro with [U-(14)C]glucose. 2. The glycosaminoglycans of corneal stroma were isolated and fractionated on cetylpyridinium chloride-cellulose columns. The major components were keratan sulphate (71%), chondroitin 4-sulphate (17%) and chondroitin 6-sulphate (4%). 3. The acid-soluble nucleotides and intermediates of glycosaminoglycan biosynthesis of corneal stroma were separated on Dowex 1 (formate form) and the tissue content and cellular concentrations were determined. 4. The rates of synthesis of the intermediates of glycosaminoglycan biosynthesis in corneal stroma were determined. 5. The incorporation of radioactivity from [U-(14)C]glucose into the uronic acid and hexosamine components of the glycosaminoglycans present in corneal stroma were measured and the turnover rates of these polymers were calculated. It was found that keratan sulphate was turning over in about 723h and chondroitin 6-sulphate in 251h.

Citing Articles

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Uridine diphosphate glucuronic acid production and utilization in various tissues actively synthesizing glycosaminoglycans.

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